PELI3、miR-744-5p在不同病理分级脑胶质瘤中的表达水平及临床意义

Expression Levels and Clinical Significance of PELI3 and miR-744-5p in Glioma with Different Pathological Grades

目的探讨不同病理分级脑胶质瘤中Pellino E3泛素蛋白连接酶家族成员3(PELI3)、微小RNA-744-5p(miR-744-5p)表达水平及意义。方法收集2016年10月至2019年10月确诊的100例脑胶质瘤患者(脑胶质瘤组)的组织标本及其临床病理资料,根据不同病理分级将脑胶质瘤组分为低级别(Ⅰ~Ⅱ级)亚组46例和高级别(Ⅲ~Ⅳ级)亚组54例;同时选取90例急性颅脑损伤患者颅内减压术切除的脑组织为对照组。采用实时荧光定量PCR(qRT-PCR)检测组织miR-744-5p表达和免疫组化学法检测PELI3蛋白表达;采用多因素Cox回归分析脑胶质瘤患者复发的影响因素。结果脑胶质瘤组PELI3蛋白阳性率显著高于对照组,miR-744-5p水平显著低于对照组(均P<0.000)。高级别组PELI3蛋白阳性率显著高于低级别组,miR-744-5p水平显著低于低级别组(均P<0.000)。PELI3、miR-744-5p表达水平与年龄、性别、肿瘤直径、KPS评分、肿瘤部位均无相关性(均P>0.05)。miR-744-5p低表达患者复发率显著高于高表达患者(P<0.05);PELI3阳性患者复发率显著高于阴性患者(P<0.05)。PELI3、病理分级是脑胶质瘤患者复发的独立危险因素,miR-744-5p则是脑胶质瘤患者复发的保护因素(P<0.05)。结论脑胶质瘤患者PELI3阳性表达升高,miR-744-5p表达水平降低,两项检测指标可作为脑胶质瘤病理分级及临床复发判定的指标。

Aim To investigate the expression levels and significance of Pellino E3 ubiquitin protein ligase family member 3(PELI3)and microRNA-744-5p(miR-744-5p)in glioma with different pathological grades.Methods The tissue samples and clinicopathologic data of 100 patients with glioma(glioma group)diagnosed in our hospital from October 2016 to October 2019 were collected.According to different pathological grades,they were divided into a low grade(Ⅰ~Ⅱgrade)group(46 cases)and a high grade(Ⅲ~Ⅳgrade)group(54 cases).At the same time,90 patients with acute brain injury were regarded as the control group.The expression of miR-744-5p in tissues was detected by real-time fluorescent quantitative PCR(qRT-PCR),the expression of PELI3 protein was detected by immunohistochemistry,multivariate Cox regression was used to analyze the influencing factors of recurrence in patients with glioma.Results The positive rate of PELI3 protein in gliomagroup was greatly higher than that in the control group,and the level of miR-744-5p was greatly lower than that in the control group(all P<0.000).The positive rate of PELI3 protein in the high grade group was greatly higher than that in the low grade group,and the level of miR-744-5p was greatly lower than that in the low grade group(P<0.000).The expression levels of PELI3 and miR-744-5p were not correlated with age,gender,tumor diameter,KPS score,and tumor site(all P>0.000).The recurrence rate of patients with low expression of miR-744-5p was greatly higher than that of patients with high expression of miR-744-5p(P<0.05);the recurrence rate of patients with positive expression of PELI3 was greatly higher than that of patients with negative expression of PELI3(P<0.05).PELI3 and pathological grade were independent risk factors for recurrence of glioma patients,and miR-744-5p was protective factor for recurrence of glioma patients(P<0.05).Conclusion The positive expression of PELI3 is increased and the expression of miR-744-5p is decreased in patients with glioma.Both of them can be used as indicators of pathological grading and clinical recurrence of glioma.