乙氧氟草醚在姜中的残留及膳食摄入风险评估

Residues and dietary risk assessment of oxyfluorfen in ginger

为探究乙氧氟草醚在姜中的最终残留风险及安全性,建立姜中的乙氧氟草醚残留检测方法,并开展240 g·L^(-1)乙氧氟草醚乳油在姜上的残留试验,获得最终残留数据,结合我国居民膳食数据及风险评估模型对慢性膳食摄入风险进行评估。结果表明,姜中的乙氧氟草醚用乙腈提取,N-丙基乙二胺(PSA)、C18及石墨化碳黑(GCB)净化,超高效液相色谱串联质谱(UPLC-MS/MS)检测,在0.01、0.05、0.1 mg·L^(-1)的添加水平下,乙氧氟草醚的添加回收率在93%~97%之间,相对标准偏差在2.6%~8.1%范围内。在山东、安徽、湖南和贵州4地,240 g·L^(-1)乙氧氟草醚乳油以180 g·hm^(-2)于播后苗前喷雾施药1次,收获期姜中乙氧氟草醚的最终残留量均低于我国及欧盟的最大残留限量值(MRL,0.01 mg·kg^(-1))。一般人群乙氧氟草醚的国家估算每日摄入量为0.0195 mg,占日允许摄入量的1.03%,姜中乙氧氟草醚的残留对慢性膳食风险的贡献仅为0.46%,表明不会对普通人群健康产生风险。研究结果为我国特色小宗作物姜中乙氧氟草醚制剂的科学合理使用提供了基础数据。

In order to explore the terminal residue and safety of oxyfluorfen in ginger,a detection method for oxyfluorfen residues in ginger was established.In addition,a residue experiment of oxyfluorfen EC on ginger was carried out,and the final residue data obtained were used to evaluate the chronic dietary intake risk based on the dietary data of Chinese residents and the risk assessment model.The oxyfluorfen was extracted with acetonitrile,purified with N-propyl ethylene-diamine(PSA),C18 and graphitized carbon black(GCB),and detected by ultra-performance liquid chromatography-tan-dem mass spectrometry(UPLC-MS/MS).At the spiked levels of 0.01,0.05,and 0.10 mg·L^(-1),the recoveries of oxyfluorfen were between 93%and 97%,with the RSDs in the range of 2.6%to 8.1%.In Shandong,Anhui,Hunan,and Guizhou prov-inces,240 g·L^(-1) oxyfluorfen EC was sprayed once with 180 g·hm^(-2) after sowing and pre-emergence.The terminal residue of oxyfluorfen in ginger during the harvest period was lower than the maximum residue limit(MRL,0.01 mg·kg^(-1))of China and European Union(EU).The NEDI of oxyfluorfen in the general population was 0.0195 mg,accounting for 1.03%of the acceptable daily intake(ADI).The contribution of oxyfluorfen residues in ginger to chronic dietary risk was only 0.46%,indicating that it would not affect the health of the general population.This study provided essential data for the scientific and rational use of oxyfluorfen on ginger,a small specialty crop in China.