背部推法对慢性疲劳综合征大鼠运动行为、氧化应激和炎症反应的影响

Effects of Tuina on motor behavior,oxidative stress and inflammatory response in rats with chronic fatigue syndrome

目的:探究背部推法对慢性疲劳综合征(CFS)大鼠运动行为、氧化应激和炎症反应的影响。方法:将24只SD大鼠按照随机数字表法分为空白组、模型组、推法组,每组8只。选用强迫负重游泳联合慢性应激刺激的方法制备CFS大鼠模型,共造模21 d。推法组在造模结束后给予背部推法,20 min/次,1次/d,连续干预14 d。记录各组大鼠一般情况半定量评分、力竭游泳时间、旷场实验运动距离。实验结束后,收集所需标本,用HE染色法观察竖脊肌组织病理学变化,并使用Image Pro Plus软件计算肌纤维横截面面积和直径,Origin软件处理绘制肌纤维横截面面积频率分布图。检测大鼠竖脊肌中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)以及血清中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6的含量。结果:(1)实验结束后,推法组大鼠一般情况半定量评分显著低于同期模型组(P<0.01)、力竭游泳时间和旷场实验运动距离均显著高于同期模型组(P<0.05,P<0.01)。(2)HE染色法结果显示,模型组大鼠竖脊肌肌细胞明显萎缩,而推法组大鼠肌细胞萎缩程度低于模型组。(3)与空白组比较,模型组大鼠竖脊肌中的SOD、GSH-Px以及PGC-1α含量均显著下降(P<0.01),血清TNF-α、IL-1β、IL-6含量显著上升(P<0.01)。与模型组比较,推法组大鼠竖脊肌SOD、GSH-Px、PGC-1α含量显著升高(P<0.05,P<0.01),血清TNF-α、IL-1β、IL-6含量显著降低(P<0.05,P<0.01)。结论:背部推法能够调节CFS大鼠的氧化应激反应,减轻炎症反应,从而改善大鼠运动行为。

Objective To explore the effect of back Tuina on motor behavior,oxidative stress and inflammation in rats with chronic fatigue syndrome(CFS).Methods Twenty-four Sprague-Dawley rats were randomly divided into a blank group,a model group and a Tuina group,each of 8,according to a random number table.The CFS rat model was prepared by means of forced weight-bearing swimming combined with chronic stress stimulation in 21 days.After modeling,the Tuina group was given daily20-minute Tuina for 14 days.The general condition semi-quantitative score,exhaustion swimming time and open field experiment(OFE)distance of all groups were recorded.After the experiment,samples were collected,and the histopathological changes of the vertical spine muscles were observed by hematoxylin and eosin(HE)staining.The cross-sectional area and diameter of muscle fibers were calculated using Image Pro Plus software,and the frequency distribution diagram of cross-sectional area of muscle fibers was processed by using the Origin software.The contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and peroxisome proliferator-activated receptorγ-coactivator 1α(PGC-1α)and tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6 in the serum of rats were measured.Results After the intervention,the general condition semi-quantitative score of the Tuina group was significantly lower than the model group(P<0.01),while the exhaustion swimming time and OFE distance were significantly higher than the latter group(P<0.05,P<0.01).(2)HE staining showed that the significant atrophy of erector spinal muscle cells in the model group,was significantly relieved in the Tuina group.(3)Compared with the blank group,the contents of SOD,GSH-Px and PGC-1αin erectus muscles decreased significantly(P<0.01),while those of TNF-α,IL-1βand IL-6 in the serum of the model group increased significantly(P<0.01).However,compared with model group,the contents of SOD,GSH-Px and PGC-1αin erectus muscle increased significantly(P<0.05,P<0.01),while those of TNF-α,IL-1βand IL-6 of the Tuina group decreased significantly(P<0.05,P<0.01).Conclusion Tuina in the back can regulate the oxidative stress response,reliever the inflammatory response and improve the motor behavior of CFS rats.