单细胞RNA测序技术在肾病中的研究进展

Advances in single-cell RNA sequencing in kidney disease

单细胞转录组测序(scRNA-seq)技术为生物医学带来了许多新发现,能高分辨率、高通量和可重复地进行转录分析,识别新的细胞类型,形成细胞图谱。而传统测序方法只能反映样本中所有细胞基因表达的整体水平而无法揭示单个细胞之间的基因表达异质性。本文阐述了scRNA-seq技术发展史,概述该技术可能应用于揭示肾脏疾病的发生机制,有利于肾病诊断和治疗。

Single-cell RNA sequencing(scRNA-seq)technology has brought many new discoveries to biomedical research,enabling high-resolution,high-throughput,and repeatable transcriptional analysis to identify new cell types and form cell maps.However,traditional sequencing methods can only reflect the overall level of gene expression of all cells in the samples and cannot reveal the heterogeneity of gene expression between individual cells.This article elaborates on the development history of scRNA-seq technology and outlines its potential application to reveal the mechanisms of kidney diseases,which is beneficial for diagnosis and treatment of kidney disease.