LncRNACRNDE、miR-4262、ZEB1在宫颈癌中的表达及与临床病理特征和预后的关系

Expression of long-chain non-coding RNA CRNDE,micro-RNA-4262 and zinc finger E-box binding homeobox 1 in cervical cancer and their relationship with clinicopathological features and prognosis

目的探讨宫颈癌患者中长链非编码RNA(LncRNA)CRNDE、微小RNA(miRNA)-4262、锌指E盒结合同源盒1(ZEB1)表达水平变化及与临床病理特征、预后的关系。方法选取2018年6月至2019年6月海南西部中心医院收治的100例宫颈癌患者作为研究对象,通过实时荧光定量聚合酶链反应法检测宫颈癌组织与癌旁组织中LncRNA CRNDE、miR-4262、ZEB1 mRNA的表达。通过在线网站预测LncRNA CRNDE与miR-4262的结合位点,miR-4262与ZEB1的结合位点,分析三者表达的相关性及与临床病理特征的关系。根据宫颈癌组织中LncRNA CRNDE、miR-4262、ZEB1 mRNA表达分为高表达组、低表达组,采用Kaplan-Meier法绘制不同LncRNA CRNDE、miR-4262、ZEB1 mRNA表达宫颈癌患者的生存曲线。结果宫颈癌组织中LncRNA CRNDE、ZEB1 mRNA表达高于癌旁组织,miR-4262表达低于癌旁组织(t=13.198、25.123、11.843,P<0.01)。宫颈癌组织中LncRNA CRNDE与miR-4262表达呈负相关,与ZEB1 mRNA表达呈正相关,miR-4262与ZEB1 mRNA表达呈负相关(r=-0.654、0.701、-0.687,P<0.01)。LncRNA CRNDE、miR-4262、ZEB1 mRNA表达与国际妇产科联盟(FIGO)分期、淋巴结转移有关(P<0.05)。随访6~36个月,失访9例,死亡13例,100例宫颈癌患者3年累积生存率为86.93%。Kaplan-Meier生存曲线结果显示,LncRNA CRNDE高表达组、miR-4262低表达组及ZEB1 mRNA高表达组3年累积生存率均较低(Log-rankχ^(2)=3.899、4.132、3.891,P<0.05)。结论宫颈癌组织中LncRNA CRNDE、ZEB1 mRNA高表达,miR-4262低表达,三者表达变化与FIGO分期、淋巴结转移和预后有关。

Objective To investigate the expression of long-chain non-coding RNA(LncRNA)CRNDE,micro-RNA(miRNA)-4262 and zinc finger E-box binding homeobox 1(ZEB1)in patients with cervical cancer and their relationship with clinicopathological features and prognosis.Methods A total of 100 patients with cervical cancer admitted to Hainan West Central Hospital from June 2018 to June 2019 were selected as study subjects.The expression of LncRNA CRNDE,miR-4262 and ZEB1 mRNA in cervical cancer tissues and paracancerous tissues by real-time fluorescence quantitative polymerase chain reaction.The binding sites of LncRNA CRNDE and miR-4262 and the binding sites of miR-4262 and ZEB1 were predicted by online website,and the correlation of their expression and their relationship with clinicopathological features were analyzed.Patients with cervical cancer were divided into high expression group and low expression group according to the expression of LncRNA CRNDE,miR-4262 and ZEB1 mRNA in cervical cancer tissues.The survival curves of patients with cervical cancer with different expression of LncRNA CRNDE,miR-4262 and ZEB1 mRNA were drawn by Kaplan-Meier method.Results The expressions of LncRNA CRNDE and ZEB1 mRNA in cervical cancer tissue were higher than those in paracancerous tissue,while the expression of miR-4262 in cervical cancer tissue was lower than that in paracancerous tissue(t=13.198,25.123,11.843;P<0.0l).The expression of LncRNA CRNDE was negatively correlated with the expression of miR-4262,positively correlated with the expression of ZEB1 mRNA,and the expression of miR-4262 was negatively correlated with ZEB1 mRNA(r=0.654,0.701,-0.687;P<0.01).The expressions of LncRNA CRNDE,miR-4262 and ZEB1 mRNA were correlated with international federation of gynecology and obstetrics(FIGO)staging and lymph node metastasis(P<0.05).During the follow-up of 6 to 36 months,9 cases lost follow-up and 13 cases died.the 3-year cumulative survival rate of 100 patients with cervical cancer was 86.93%.The results of Kaplan-Meier survival curve showed that the 3-year cumulative survival rate was lower in LncRNA CRNDE high expression group,miR-4262 low expression group and ZEB1 mRNA high expression group(Log-rankχ^(2)=3.899,4.132,3.891;P<0.05).Conclusions LncRNA CRNDE and ZEB1 mRNA are highly expressed and miR-4262 is lowly expressed in cervical cancer tissues.The expression changes of the three are related to FIGO stage,lymph node metastasis and prognosis.