宫颈鳞状上皮内病变和宫颈癌Th17、HIF-1α水平变化与HPV感染的关系

Relationship between changes in T helper cell 17,hypoxic induction factor-1αlevels and human papilloma virus infection in cervical squamous intraepithelial lesion and cervical cancer

目的探讨宫颈鳞状上皮内病变(SIL)和宫颈鳞状细胞癌(SCC)辅助性T细胞17(Th17)、缺氧诱导因子-1α(HIF-1α)水平变化与人乳头瘤病毒(HPV)感染的关系。方法收集2020年1月至2023年1月于金寨县人民医院病理科就诊的40例宫颈SIL患者(SIL组)、40例宫颈SCC患者(SCC组)的病理组织和静脉血指标,并收集同期同一医院20例行全子宫切除的患者(对照组)的正常宫颈上皮组织和静脉血指标。对三组Th17、HIF-1α水平及HPV阳性感染率进行检测并比较。结果SCC组和SIL组Th17、HIF-1α水平显著高于对照组(P<0.05),且SCC组高于SIL组(P<0.05)。SCC组和SIL组HPV单一、多重感染率比较无统计学差异(P>0.05);HPV感染患者的Th17、HIF-1α水平均显著高于未感染患者(P<0.05),且多重感染患者的Th17和HIF-1α水平显著高于单一感染患者(P<0.05)。Th17、HIF-1α水平与HPV感染呈显著相关(P<0.05),且两种指标之间亦存在显著相关(P<0.05)。结论Th17、HIF-1α水平可作为SCC患者预后的客观预测指标及指导临床治疗的参考。

Objective To discuss the relationship between changes in T helper cell 17(Th17),hypoxic induction factor-1α(HIF-1α)and human papilloma virus(HPV)infection in cervical squamous intraepithelial lesion(SIL)and cervical squamous cell carcinoma(SCC).Methods The pathological tissues and venous blood of 40 patients with cervical SIL(SIL group)and 40 cervical SCC patients(SCC groups)from the Pathology Department of Jinzhai County People′s Hospital from January 2020 to January 2023 were collected,and the normal cervical epithelium tissue and venous blood of 20 patients(control group)with general uterine resection were collected in the same hospital during the same period.Th17,HIF-1αlevels and HPV-positive infection rates were detected and compared among the three groups.Results Th17 and HIF-1αlevels in SCC groups and SIL group were significantly higher than the control group(P<0.05),and the SCC group was higher than the SIL group(P<0.05).There were no statistical differences between SCC group and SIL group in single and multiple HPV infection rates(P>0.05).Th17 and HIF-1αlevels in HPV infection patients were significantly higher than those in uninfected patients(P<0.05),and Th17 and HIF-1αwere significantly higher in patients with multiple infection than patients with single infected(P<0.05).Th17 and HIF-1αlevels were significantly correlated with HPV infections(P<0.05),and there are significant correlation between the two indicators(P<0.05).Conclusions Th17 and HIF-1αlevels can be used as the objective prediction indicators for SCC patients and references for clinical treatment.