卵巢癌患者外周血HDAC2、PD-L1蛋白及调节性T细胞水平与临床病理特征和预后的关系分析

Analysis of the relationship between peripheral blood histone deacetylase 2,programmed death ligand 1 protein,and regulatory T-cell levels with clinicopathological features and prognosis in patients with ovarian cancer

目的探讨卵巢癌(OC)患者外周血组蛋白脱乙酰基酶(HDAC2)、程序性死亡配体1(PD-L1)蛋白及调节性T细胞(Treg)水平与临床病理特征和预后的关系。方法选取2018年7月至2019年9月在张家口市第一医院确诊治疗的60例OC患者作为研究组,根据随访3年患者生存情况分为生存组(n=43)、死亡组(n=17);另选取同期同一医院确诊的60例卵巢良性肿瘤患者作为对照组。采用流式细胞术检测外周血Treg、辅助性T细胞17(Th17)和Th17/Treg水平,采用酶联免疫吸附试验法检测HDAC2和PD-L1蛋白水平。分析Treg、Th17、Th17/Treg、HDAC2和PD-L1蛋白水平与临床病理特征间的关系;治疗后随访3年,分析比较生存组和死亡组患者外周血Treg、Th17、Th17/Treg、HDAC2和PD-L1蛋白水平;采用Logistic回归分析OC患者预后的影响因素。结果研究组患者Treg、Th17、Th17/Treg、HDAC2和PD-L1水平均显著高于对照组(P<0.05);OCⅠ期、Ⅱ期、Ⅲ期和Ⅳ期患者Treg、Th17、Th17/Treg、HDAC2和PD-L1蛋白水平均依次升高,差异具有统计学意义(P<0.05)。临床分期、残存病灶大小、淋巴结转移、Treg、Th17、Th17/Treg、HDAC2和PD-L1蛋白水平均为OC患者预后的影响因素(P<0.05)。结论Treg、Th17、Th17/Treg、HDAC2和PD-L1蛋白参与并影响OC的发展,与患者预后密切相关。

Objective To investigate the relationship between the detection of peripheral blood histone deacetylase 2(HDAC2)and programmed death ligand 1(PD-L1)protein and regulatory T-cell(Treg)detection with clinicopathological features and prognosis in patients with ovarian cancer.Methods A total of 60 OC patients diagnosed and treated at Zhangjiakou First Hospital from July 2018 to September 2019 were selected as the study group.The patients were grouped into survival group(n=43)and death group(n=17)according to the 3-year follow-up of patients′survival condition.Another 60 patients with benign ovarian tumors diagnosed in the same hospital during the same time were selected as the control group.The levels of Treg,T helper cell 17(Th17)and Th17/Treg level in peripheral blood of all patients were detected by flow cytometry,the levels of HDAC2 and PD-L1 proteins were detected by enzyme-linked immunosorbent assay.The relationship between the levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins and the clinicopathological features was analyzed,the peripheral blood Treg,Th17,Th17/Treg,HDAC2,and PD-L1 protein levels were analyzed and compared between the survival group and the death group after follow-up for 3 years after treatment.Logistic regression was applied to analyze the factors influencing the prognosis of OC patients.Results The levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 in patients in the study group were significantly higher than those in the control group(P<0.05).The levels of Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins in OC patients in stage I,stage II,stage III and stage IV increased successively,and the difference was statistically obvious(P<0.05).The clinical stage,residual lesion size,lymph node metastasis,Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins levels were all influencing factors for the prognosis of OC patients(P<0.05).Conclusions Treg,Th17,Th17/Treg,HDAC2 and PD-L1 proteins participate in and affect the development of OC,which are closely related to the prognosis of patients.