黏蛋白MUC3,MUC4在胆管癌中的表达及其临床意义

Expression and clinical significance of mucins MUC3 and MUC4 in cholangiocarcinoma

目的研究MUC3,MUC4在CCA中的表达及其临床意义。方法选取2017年1月~2022年12月我院手术的68例CCA手术切除的新鲜标本设为癌症组,同时选取18例健康人正常胆管组织标本设为对照组。应用SP免疫组化法对组织切片进行染色,测定MUC3,MUC4,并分析在其CCA中的表达及其临床病理特征的关系。结果①MUC3在CCA组织:癌症组阳性率60.29%,对照组11.11%(P<0.01);MUC4在CCA组织:癌症组阳性率58.82%,对照16.67%(P<0.01)。②MUC3在有转移的癌症组中表达的平均秩为42.26,无转移25.49(P<0.01);MUC4在有转移的癌症组中表达的平均秩43.15,无转移26.45(P<0.01)。结论通过对MUC3,MUC4的测定,发现其在CCA组织中明显升高,有转移较无转移的也明显升高,可能与它可以引起细胞游走和黏附的异常,参与CCA的侵袭与转移。

Objective:To study the expression of MUC3 and MUC4 in CCA and its clinical significance.Methods From Janu-ary 2017 to December 2022,68 fresh CCA specimens were selected as cancer group,and 18 normal bile duct specimens were selected as control group.The t issue sections were stained with SP immunohistochemistry to determine the expression of MUC3 and MUC4 in CCA and to analyze the relationship between their clinicopathological features.Results The positive rate of MUC3 in CCA tissues was 60.29%in cancer group and 11.11%in control group(P<0.01).The positive rate of MUC4 in CCA tis-sues was 58.82%in cancer group and 16.67%in control group(P<0.01).The mean rank of MUC3 expression in cancer group with metastasis was 42.26 and 25.49 without metastasis(P<0.01).The mean rank of MUC4 expression in the cancer group with metastasis was 43.15 and 26.45 without metastasis(P<0.01).Conclusion Through the determination of MUC3 and MUC4,it was found that the levels of MUC3 and MUC4 in CCA tissues were significantly increased,and those with metastasis were also signif-icantly increased compared with those without metastasis,which may be related to the abnormal cell migration and adhesion,