摘要
【目的】深入解析大豆开花期和成熟期的遗传基础,发掘控制性状的重要基因组区间,为分子标记辅助选择育种和新基因克隆提供依据。【方法】以224份携带显性E1等位基因的大豆种质为试验材料,在4个种植环境下调查表型数据,用R-mrMLM软件包中的6种多位点关联分析模型对大豆开花和成熟期进行全基因组关联分析。【结果】共检测到91个开花期和83个成熟期QTNs(quantitative trait nucleotides),其中6个开花期和10个成熟期QTNs能在多环境中检测到,这些环境稳定QTNs分布于15个区间大小在90~490 kb的单倍型(基因组)内,并新检测到6个基因组区间。【结论】显性E1等位基因背景下大豆开花和成熟期的QTN构成复杂,位于5号染色体39.52~40.01 Mb等15个基因组区间是该群体中控制大豆开花期或成熟期的重要位点。
[Objective]The objectives of this study are to dissect the genetic basis of soybean flowering and maturity time and to identify genomic regions that can be used for molecular marker-assisted selection(MAS)and novel gene cloning.[Methods]224 soybeans with dominant E1 alleles were planted in four environments to investigate phenotypic data.Six multi-locus association analysis models from the R-mrMLM software package were employed for a genome-wide association analysis(GWAS)on soybean flowering and maturity time.[Results]91 flowering and 83 maturation QTNs(quantitative trait nucleotides)were detected by combining six multi-loci GWAS methods.Among them,6 flowering and 10 maturation QTNs were detected in various environments.These stable QTNs were distributed in 15 haplotype blocks(genomic regions)with an interval size of 90-490 kb.6 out of 15 genomic regions were newly detected.[Conclusion]The QTN composition of soybean flowering and maturity time at the dominant E1 gene back-ground is complex.15 genomic regions,including 39.52-40.01 Mb on chromosome 5,are loci control-ling soybean flowering or maturity time in this population.
作者
张普
贾世豪
莎珍
杨植雅
欧侨欣
曹永策
ZHANG Pu;JIA Shihao;SHA Zhen;YANG Zhiya;OU Qiaoxin;CAO Yongce(College of Life Science,Yanan University/Shaanxi Key Laboratory of Chinese Jujube,Yanan,Shaanxi 716000,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第4期624-633,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(32160469)
陕西省自然科学基础研究计划项目(2020JQ-795)
陕西省科协青年人才托举计划项目(20200205)
延安大学校级科研计划项目(YDY2020-30)
陕西省大学生创新创业训练计划项目(S202310719041,S202210719092)。