文冠果油对雄性小鼠记忆能力的影响

Effect of Xanthoceras sorbifolium Bunge.oil on memory ability of male mice

为了对文冠果油相关产品的开发提供数据支撑,将健康雄性小鼠分为4个组,即对照组(玉米油)、文冠果油低剂量组〔2.1 g/(kg·d)〕、文冠果油中剂量组〔4.2 g/(kg·d)〕和文冠果油高剂量组〔12.5 g/(kg·d)〕,分别经口给予小鼠灌胃1个月后,测定小鼠体质量,并进行跳台试验、避暗试验、水迷宫试验以评价文冠果油对雄性小鼠记忆能力的影响,试验结束后测定小鼠海马和大脑皮质组织匀浆中多巴胺(DA)、5-羟色胺(5-HT)、去甲肾上腺素(NE)、乙酰胆碱(ACh)含量及乙酰胆碱酯酶(AChE)活性,探讨文冠果油对小鼠记忆能力的影响机制。结果表明:经过1个月的灌胃试验,各组小鼠体质量及其增长率均无显著差异;文冠果油组小鼠跳台试验、避暗试验、水迷宫试验均为阳性;文冠果油高剂量组小鼠海马和大脑皮质组织匀桨中5-HT、ACh、NE、DA含量明显升高,AChE活性明显降低。综上,文冠果油具有改善小鼠记忆的功能,且是通过改善单胺类神经递质和胆碱能神经递质的代谢发挥作用的。

In order to provide data support for the development of Xanthoceras sorbifolium Bunge.oil related products,healthy male mice were divided into 4 groups,namely control group(corn oil),low-dose group〔2.1 g/(kg·d)〕,medium-dose group〔4.2 g/(kg·d)〕and high-dose group〔12.5 g/(kg·d)〕of the Xanthoceras sorbifolium Bunge.oil.After one month of intragastric administration,the body weight of mice was measured,and the step-down test,dark avoidance test and water maze test were carried out to evaluate the effects of Xanthoceras sorbifolium Bunge.oil on memory ability of male mice.In addition,the contents of dopamine(DA),5-hydroxytryptamine(5-HT),norepinephrine(NE),acetylcholine(ACh)and the activity of acetylcholinesterase(AChE)in the homogenates of hippocampus and cerebral cortex of mice were measured to explore the mechanism of the effect of Xanthoceras sorbifolium Bunge.oil on memory ability of mice.The results showed that there was no significant difference in body weight and growth rate among all groups after one month of intragastric administration test.The step-down test,dark avoidance test and water maze test were all positive.In high-dose group,the contents of 5-HT,ACh,NE and DA in homogenates of hippocampus and cerebral cortex of mice were significantly increased and the activity of AChE was significantly decreased.In summary,Xanthoceras sorbifolium Bunge.oil has the function of improving memory of mice,and it plays the role by improving the metabolism of monoamine neurotransmitters and cholinergic neurotransmitters.