猪胚胎移植技术在预防猪圆环病毒2型感染中的应用

The application of pig embryo transfer technology in the prevention of porcine circovirus type 2 infection

试验旨在研究猪胚胎移植技术在预防猪圆环病毒2型感染中的应用,为猪群的健康养殖提供参考。选取健康猪卵巢,挑选细胞质均匀的卵丘-卵母细胞复合体,培养并获取成熟卵母细胞。选择体重大于30 kg、处于正常发情期的成熟健康母猪,将成熟卵母细胞移植入母猪体内,并回收其早期胚胎。利用猪圆环病毒2型ELISA抗原检测试剂盒判别健康猪胚胎的防疫效果。结果表明:健康猪胚胎至少能够防疫浓缩3~4倍浓度的猪圆环病毒2型,防疫效果较好。说明猪胚胎移植技术能够在胚胎水平上建立健康种群,有效预防猪圆环病毒2型感染仔猪。

The aim of this experiment was to study the application of porcine embryo transfer technology in prevention of porcine circovirus type 2 infection,so as to provide a reference for pig production.Healthy pig ovaries for the experiment were collected,the cumulus oocyte complex with uniform cytoplasm was picked up and cultured to obtain mature oocytes.The mature healthy recipient sows with a weight of greater than 30 kg and in normal estrus were chosen and had mature oocytes transferred into the uterine to recover their early embryos.Using the Porcine Circovirus Type 2 ELISA Antigen Detection Kit to determine the epidemic prevention effect of healthy porcine embryos.The results showed that healthy porcine embryos could prevent the 3~4 folds concentration of porcine circovirus type 2 at least,showing an encouraging epidemic prevention effect.It could be concluded that the porcine embryo transfer technology could help establish a healthy population on embryo levels,and effectively prevent porcine circovirus type 2 infection in piglets.