接触氧化除铁除锰工艺中微生物的协同作用

Study on the synergistic mechanism of microorganisms in the contact oxidation iron and manganese removal process

以辽宁农村地区二个典型接触氧化除铁除锰水厂为研究对象,采用16S rRNA高通量测序技术对工艺各单元的微生物群落结构进行分析.结果发现,接触氧化除铁除锰工艺各处理单元中的微生物群落结构显著不同,Proteobacteria(43.86%)是接触氧化除铁除锰工艺中的优势菌门,Gallionella和Flavobacterium是主要的除铁锰功能菌属.相关性分析结果表明,Rhodoferax(0.71%)和Sulfurospirillum(0.75%)等菌属与菌落总数,铁和锰浓度之间呈显著正相关关系,说明尽管其相对丰度较低,但仍在系统中发挥着不可或缺的作用.通过分子生态网络与拓扑特性的深入分析以及COGs功能预测,揭示了Gallionella,Flavobacterium,Pseudomonas和Rhodoferax等菌属间的协同作用关系.不同处理单元对微生物群落结构产生了显著影响,但对这些微生物的代谢影响较小,表明形成的微生物共存系统相对稳定,对水中铁锰离子的高效稳定去除有着重要作用.

This study focused on two typical contact oxidation iron and manganese removal water treatment plants in rural areas of Liaoning province.The microbial community structure during the process was analyzed using 16S rRNA high-throughput sequencing technology.The results indicated that the contact oxidation iron and manganese removal process could significantly alter the microbial community structure in the source water.Proteobacteria(43.86%)was the dominant phylum in the process.Gallionella and Flavobacterium were identified as the major iron and manganese functional genera.Correlation analysis revealed that even at relatively low abundance,genera like Rhodoferax(0.71%)and Sulfurospirillum(0.75%)showed significant positive correlations with colony counts,iron and manganese concentrations,indicating their important roles in this system.In-depth analysis using molecular ecological networks and topological properties,along with COGs functional predictions,revealed synergistic metabolic relationships among genera like Gallionella,Flavobacterium,Pseudomonas and Rhodoferax.Despite the influence of different treatment stages on microbial community structure,their metabolic effects remained relatively small,indicating the formation of a relatively stable coexistence system that plays an important role in the efficient and stable removal of iron and manganese ions in water.