基于肠道菌群-短链脂肪酸探讨宣肺散结汤对肺癌小鼠肿瘤抑制与免疫功能的影响

Exploration of the effects of Xuanfei Sanjie decoction on tumor inhibition and immune function in lung cancer mice based on intestinal flora--short-chain fatty acids

目的 基于肠道菌群-短链脂肪酸(short-chain fatty acids,SCFAS)探讨宣肺散结汤对肺癌小鼠肿瘤抑制及免疫功能的影响机制。方法 选取C57BL/6雄性小鼠,建立肺癌小鼠模型。取32只造模成功小鼠,随机分为模型组(等体积的0.9%NaCl溶液灌胃)、顺铂组(腹腔注射顺铂溶液0.1 mg·d^(-1)+等体积的0.9%Na Cl溶液灌胃)、宣肺散结汤组(宣肺散结汤灌胃0.6 mL·d^(-1))和联合组(腹腔注射顺铂溶液0.1 mg·d^(-1)+宣肺散结汤灌胃0.6 mL·d^(-1)),每组8只。另取8只健康C57BL/6雄性小鼠作为正常对照组。连续灌胃给药14 d后进行指标检测。结果 模型组和顺铂组均出现摄食量减少、脱毛、反应迟钝等现象;宣肺散结汤组及联合组摄食量可,脱毛较少,反应较灵敏。与模型组比较,顺铂组、宣肺散结汤组、联合组瘤质量均减轻,其中联合组瘤质量最轻,抑瘤率最高(P<0.05);与模型组比较,顺铂组胸腺指数、脾脏指数、Chao1、shannon均下降,宣肺散结汤组和联合组上述指标均升高,其中联合组更高(P<0.05);与模型组比较,顺铂组、宣肺散结汤组、联合组CD4^(+)、CD4^(+)/CD8^(+)和戊酸、己酸含量均上升,CD8^(+)和乙酸、丙酸含量均下降,其中联合组CD4^(+)、CD4^(+)/CD8^(+)和戊酸、己酸含量最高,CD8^(+)和乙酸、丙酸含量最低(P<0.05)。结论 宣肺散结汤可通过改善肠道菌群和SCFAs代谢提高肺癌小鼠免疫功能,抑制肿瘤生长。

Objective To explore the mechanism of the effect of Xuanfei Sanjie decoction on tumour inhibition and immune function in lung cancer mice based on intestinal?ora--short-chain fatty acids(SCFAs).Methods C57BL/6 male mice were selected to establish a mouse model of lung cancer.Thirty-two successfully modeled mice were selected and randomly divided into the model group(equal volume of 0.9%NaCl solution by gavage),the cisplatin group(intraperitoneal injection of cisplatin solution0.1 mg·d^(-1)+equal volume of 0.9%NaCl solution by gavage),the Xuanfei Sanjie decoction group(Xuanfei Sanjie decoction by gavage 0.6 mL·d^(-1))and the combination group(intraperitoneal injection of cisplatin solution 0.1 mg·d^(-1)+Xuanfei Sanjie decoction by gavage 0.6 mL·d^(-1)),with 8 mice in each group.Another eight healthy C57BL/6 male mice were selected as the normal control group.The indexes were tested after 14 days of continuous gavage administration.Results The model group and the cisplatin group showed reduced intake,hair loss and unresponsiveness;the Xuanfei Sanjie decoction group and the combination group had acceptable intake,less hair loss and the more sensitive response.Compared with the model group,the tumour mass was reduced in the cisplatin group,the Xuanfei Sanjie decoction group and the combination group,among which the combination group had the lightest tumour mass and the highest tumour inhibition rate(P<0.05).Compared with the model group,the thymus index,spleen index,Chao1 and shannon decreased in the cisplatin group,and the above indexes increased in the Xuanfei Sanjie decoction group and the combination group,among which the combination group had the higher value(P<0.05).Compared with the model group,the CD4^(+),CD4^(+)/CD8^(+)and valeric acid and caproic acid contents in the cisplatin group,the Xuanfei Sanjie decoction group and the combination group increased,and the CD8^(+)and acetic acid and propionic acid contents decreased,with the combination group having the highest contents of CD4^(+),CD4^(+)/CD8^(+)and valeric acid and caproic acid,and the lowest contents of CD8^(+)and acetic acid and propionic acid(P<0.05).Conclusion Xuanfei Sanjie decoction can improve immune function and inhibit tumour growth in lung cancer mice by improving intestinal?ora and SCFAs metabolism.