DHCR7基因在胃癌中的表达及其与免疫相关基因的关系

Expression of DHCR7 Gene in Gastric Cancer and Its Relationship with Immune-Related Genes

目的探究7-脱氢胆固醇还原酶(DHCR7)基因在胃癌中的表达及其与免疫相关基因的关系。方法使用UALCAN、TIMER数据库分析DHCR7基因在不同类型肿瘤中的表达情况。使用GEPIA、TCGA、GEO数据库分析DHCR7基因在胃癌中的表达。Kaplan-Meier Plotter数据库分析DHCR7基因表达与胃癌患者预后的相关性。通过cBioPortal数据库找出与DHCR7共表达的基因,并展示这些基因中相关系数比较高的基因。对胃癌中与DHCR7表达正负相关的基因进行基因本体分析(GO)和京都基因与基因组百科全书(KEGG)分析,首先分析DHCR7基因与CD8^(+)T细胞以及招募CD8^(+)T细胞相关趋化因子的相关性;其次分析DHCR7基因与免疫激活相关基因的相关性;最后分析DHCR7表达与胃癌患者临床病理学特征的关联。结果DHCR7基因在大多数肿瘤类型中均有高表达趋势。DHCR7基因在胃癌中的表达高于正常胃黏膜。Kaplan-Meier Plotter数据库中201790-s-at芯片结果显示高表达DHCR7组患者生存期较短。与DHCR7负相关基因的GO、KEGG通路富集分析主要富集在免疫相关功能和信号通路上,DHCR7与CD8^(+)T细胞、招募CD8^(+)T细胞相关的趋化因子以及免疫激活基因都具有负相关性。DHCR7高表达与胃癌患者年龄呈正相关。结论DHCR7在胃癌组织中呈高表达,高表达DHCR7患者预后不良,DHCR7基因能够影响胃癌免疫微环境,有望成为胃癌免疫治疗的新靶点。

Objective To explore the expression of 7-dehydrocholesterol reductase(DHCR7)gene in gastric cancer and its relationship with immune-related genes.Methods UALCAN and TIMER databases were used for analyzing the expression of DHCR7 gene in different types of tumors.GEPIA,TCGA and GEO databases were used for investigating the expression of DHCR7 gene in gastric cancer.Kaplan-Meier Plotter database was used for evaluating the relationship between DHCR7 gene expression and the prognosis of gastric cancer patients.cBioPortal database was used for identifying the co-expressed genes with DHCR7 in gastric cancer,and the genes with higher correlation coefficients among the co-expressed genes were displayed.Geneontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis were performed to analyze the co-expressed gene with DHCR7.The correlation between DHCR7 gene and CD8^(+)T cells,chemokines related to recruitment of CD8^(+)T cells,as well as immune activation related-genes were evaluated.The relationship between the expression of DHCR7 and clinical pathological feature of gastric cancer patients were classified.Results DHCR7 gene had a high expression trend in most tumor types.The expression of DHCR7 gene in gastric cancer was higher than that in normal gastric mucosa.The results of 201790-s-at chip in Kaplan-Meier Plotter database showed that the survival of patients with high expression of DHCR7 was shorter.The GO and KEGG pathway enrichment analysis of negatively correlated genes with DHCR7 were mainly enriched in immune-related functions and signaling pathways.DHCR7 had a negative correlation with CD8^(+)T cells,chemokines associated with recruiting CD8^(+)T cells,and immune activation related-genes.The high expression of DHCR7 was positively correlated with the age of gastric cancer patients.Conclusion DHCR7 is highly expressed in gastric cancer tissue,and patients with high expression of DHCR7 have poor prognosis.The DHCR7 gene can affect the immune microenvironment of gastric cancer and is expected to become a new target for immunotherapy of gastric cancer.