银杏种仁分离蛋白水解产物的制备及其抗氧化活性研究

Preparation of Hydrolysates from Ginkgo biloba Seed Protein Isolate and Their Antioxidant Activity

采用碱性蛋白酶、菠萝蛋白酶和米曲霉蛋白酶对银杏种仁分离蛋白(GBSPI)进行水解,并通过体外DPPH自由基、羟基自由基和超氧阴离子自由基清除实验评价GBSPI水解产物的抗氧化活性。研究结果表明:碱性蛋白酶、菠萝蛋白酶和米曲霉蛋白酶水解产物的得率分别为93.60%、60.00%和46.80%,水解度分别为13.76%、6.28%和5.55%。3种酶水解产物对DPPH自由基、羟基自由基和超氧阴离子自由基均具有较好的抑制作用,其中,碱性蛋白酶水解产物具有最强的抗氧化活性,其对DPPH自由基、羟基自由基和超氧阴离子自由基的IC 50值分别为5.29、0.35和7.01 g/L。

Ginkgo biloba seed protein isolate(GBSPI)was hydrolyzed by alcalase,bromelain,and Aspergillus oryzae protease,and the antioxidant activity of the hydrolysates was evaluated through in vitro DPPH radical,hydroxyl radical,and superoxide anion radical scavenging experiments.The results showed that the yields of hydrolysates by alcalase,bromelain,and Aspergillus oryzae protease were 93.60%,60.00%and 46.80%,with degrees of hydrolysis were 13.76%,6.28%and 5.55%,respectively.The hydrolysates from three enzymes exhibited good inhibitory effects on DPPH radical,hydroxyl radical,and superoxide anion radical.Among them,the hydrolysate from alcalase showed the strongest antioxidant activity,with IC 50 values of 5.29 g/L for DPPH radical,0.35 g/L for hydroxyl radical,and 7.01 g/L for superoxide anion radical,respectively.