运前密度和温度驯化对斑点叉尾鮰幼鱼模拟运输的影响

Effects of pre-transport density and temperature domestication on simulated transport of juvenile Ictalurus punctatus

探究了不同运前驯化方式对斑点叉尾鮰(Ictalurus punctatus)幼鱼运输过程中水质、生化参数及组织结构的影响,为其健康运输提供参考依据。通过控制密度(鱼水质量比分别为1∶2、1∶3、1∶4)和温度(14、19、24℃)两个条件对其先后进行运前密集驯化和温度驯化,随后模拟运输18 h,并考察模拟运输过程中(第0、第2、第6、第18小时)和运后恢复24 h的水质(氨氮、pH)、生化参数[葡萄糖(Glu)、皮质醇(Cor)、乳酸脱氢酶(LDH)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、丙二醛(MDA)]以及组织结构变化(皮肤、肠道)。结果显示,运前驯化密度1∶4、温度19℃处理组的氨氮显著低于其他实验组,pH显著高于其他实验组(P<0.05),Glu、Cor、LDH、CAT、SOD和MDA优于其他实验组,并且皮肤和肠道结构损伤程度最低。研究表明,运前驯化密度1∶4、温度19℃能够有效提高斑点叉尾鮰幼鱼在有水运输过程中的抗应激能力。

To explore the effects of different pre-transport acclimation methods on water quality,biochemical parameters and tissue structure of juvenile Ictalurus punctatus during transportation,and to provide references for transportation of juvenile I.punctatus,we conducted pre-transport intensive acclimation and temperature acclimation.The pre-transport intensive acclimation involved fish-to-water ratios of 1∶2,1∶3 and 1∶4,and the temperature acclimation was at 14,19 and 24℃.The simulation transport lasted for 18 h,and we investigated the changes in water quality(Ammonia nitrogen,pH),biochemical parameters[Glucose(Glu)、cortisol(Cor),lactate dehydrogenase(LDH),catalase(CAT),superoxide dismutase(SOD),malondialdehyde(MDA)]as well as organizational structure(Skin and intestine)at different time(0th,2nd,6th,18th hour)and 24-hour recovery after transport.The results show that pre-transport intensive acclimation with a fish-to-water ratio of 1∶4 and a temperature of 19℃ had lower ammonia nitrogen levels than the other treatments(P<0.05).Glu,Cor,LDH,CAT,SOD and MDA levels were higher than the other groups,and there was minimal damage to the skin and intestinal structures.In conclusion,pre-transport intensive acclimation with a fish-to-water ratio of 1∶4 and a temperature of 19℃ can improve anti-stress capacity effectively during transportation of juvenile I.punctatus.