6个接骨木优良品系耐盐性综合评价

Comprehensive Evaluation of Salt Tolerance of 6 Excellent Strains of Sambucus williamsii

为揭示接骨木新品系对盐胁迫的适应能力,筛选耐盐能力更强的接骨木新品系,采用盆栽法,以6个接骨木品系1年生扦插苗为试验材料,通过不同浓度NaCl(0‰、2‰、4‰、6‰)胁迫处理,分析比较处理后6个品系之间的生长、生理指标差异,并对指标进行主成分综合分析评价。结果表明:随着NaCl处理浓度的升高,6个接骨木品系的苗高增量呈先上升后下降的趋势,植株鲜质量、干质量与植株整体健康状况呈现逐渐下降的趋势,6‰NaCl处理的健康状况较0‰下降幅度最小的为‘水丹’,降幅为20.69%;6个接骨木品系的丙二醛含量、细胞膜透性呈现逐渐上升的趋势,‘水丹’的丙二醛含量上升幅度最小;6个接骨木品系叶片的叶绿素含量、可溶性蛋白含量、叶片相对含水量呈现逐渐下降的趋势,其中‘盐丹’和‘水丹’的叶绿素含量均高于其他品系,‘盐丹’的可溶性蛋白含量和叶片相对含水量下降幅度最小,6‰NaCl浓度处理较0‰降幅分别为45.08%和2.30%;6个接骨木品系的超氧化物歧化酶活性和过氧化物酶活性均呈现先上升后下降的趋势,0‰、2‰、4‰NaCl浓度下,‘水丹’的过氧化物酶活性显著低于‘红丰’‘紫丰’。对以上指标进行双因素方差分析得出,品系和盐浓度的交互作用显著影响接骨木的植株整体健康状况、叶片相对含水量、干质量。综合分析得出6个接骨木品系耐盐能力为‘水丹’>‘盐丹’>‘紫丰’>‘红丰’>‘金幻’>‘柳叶红’。‘水丹’和‘盐丹’耐盐性较强,可作为盐碱地绿化候选树种。

In order to reveal the adaptability of new strains of Sambucus williamsii to salt stress and screen for new strains with stronger salt tolerance,this experiment used pot culture method and 1-year old cutting seedlings of 6 Sambucus williamsii strains as experimental materials.Different concentrations of NaCl(0‰,2‰,4‰,6‰)were applied to analyze and compare the differences in growth and physiological indexes between the 6 strains after treatment,and to conduct principal component comprehensive analysis and evaluation.The results showed that with the increase of NaCl concentration,the seedling height increment of 6 Sambucus williamsii strains increased firstly and then decreased,and the fresh weight,dry weight and overall health status of the plants showed a gradual decline.The health status of 6‰NaCl treatment decreased the least by 20.69%compared with 0‰NaCl treatment of‘Shuidan’.The content of malondialdehyde and cell membrane permeability of the 6 Sambucus williamsii strains showed a gradually increasing trend,while the content of malondialdehyde in‘Shuidan’showed the smallest increase compared to the control with 0‰NaCl.The chlorophyll content,soluble protein content and the relative water content of the leaves of 6 Sambucus williamsii strains showed a gradually decreasing trend,among them,the chlorophyll content of‘Yandan’and‘Shuidan’was higher than that of other strains,and the soluble protein content and the relative water content of‘Yandan’showed the smallest decrease,and the 6‰NaCl treatment decreased by 45.08%and 2.30%compared with 0‰NaCl treatment,respectively.The superoxide dismutase activity and peroxidase activity of the 6 Sambucus williamsii strains showed a tendency of increasing and then decreasing,and the peroxidase activity of‘Shuidan’was significantly lower than that of‘Hongfeng’and‘Zifeng’at the concentrations of 0‰,2‰,and 4‰NaCl.By conducting a bivariate analysis of variance on the above indexes,it was found that the interaction between strain and salt concentration significantly affected the overall health status of the plant,leaf relative water content,and dry weight of Sambucus williamsii plants.Through comprehensive analysis,it was found that the salt tolerance of the 6 Sambucus williamsii strains was‘Shuidan’>‘Yandan’>‘Zifeng’>‘Hongfeng’>‘Jinhuan’>‘Liuyehong’,‘Shuidan’and‘Yandan’have strong salt tolerance and can be used as candidate tree species for saline-alkali land greening.