靶向编辑O⁃GlcNAc糖基化修饰的化学生物学技术

Chemical Biology Techniques for Targeted Manipulation of O-GlcNAcylation

糖基化修饰是最丰富和最复杂的蛋白质翻译后修饰之一。其中,氧连-N-乙酰氨基葡萄糖基化修饰(O-GlcNAcylation)作为广泛存在于真核细胞质或线粒体的蛋白质糖修饰,影响了蛋白质性质、细胞功能和疾病状态等方面,因此,在活细胞中的靶蛋白质上进行编辑O-GlcNAc糖基化对于与其相关的功能调控至关重要。这些在细胞靶蛋白质上操控O-GlcNAc糖基化修饰的新技术,将在很大程度上加速O-GlcNAc功能研究的发展。本文简要介绍了近年来靶向编辑O-GlcNAc糖基化修饰化学生物学技术的研究进展,讨论了目前可用的O-GlcNAc编辑策略并进行了分析,展望了相关技术的未来发展前景。这些技术组成了一个强大的化学生物学工具箱,并有望用于与O-GlcNAc糖基化相关疾病的诊断与治疗。

O-linked-N-acetylglucosaminylation(O-GlcNAcylation)is a type of glycosylation that widely present in eukaryotic cytoplasmic proteins or mitochondria.It affects protein properties,cell functions,disease states and so on,hence it is import to identify the roles that O-GlcNAcylations play on target proteins in living cells.The new technologies for manipulating O-GlcNAcylations on target proteins will greatly accelerate the understanding of O-GlcNAc’s functions.This article briefly introduces some recent research progress of chemical biology techniques for targeted protein O-GlcNAcylation,and also analyzes the advantages and limitations of these strategies,and their future development prospects.These technologies build a powerful chemical biology toolbox,which may contribute to the diagnoses and treatments related to O-GlcNAcylation.