UCP1基因多态性与糖尿病患者周围血管病变的相关性研究

Study on the Correlation Between UCP1 Gene Polymorphism and Peripheral Vascular Disease in Patients with Diabetes Mellitus

目的探讨UCP1基因多态性与糖尿病患者发生周围血管病变的关系。方法以2019年7月-2020年7月我院440例糖尿病患者为研究对象,踝臂指数≤0.9为周围血管病变组,共207例;踝臂指数在0.9~1.4为对照组,共233例。采全血后提取DNA,SNaPshot法检测UCP1基因多态性,分析其与围血管病变关系及常见危险因素,MDR软件分析基因与环境因素交互作用。结果两组BMI、SBP、DBP、TG、TC、LDL及NA比较,差异无统计学意义(P>0.05);两组病程、FBS、HbA1c、HDL及吸烟史比较,差异有统计学意义(P<0.05)。糖尿病病程长和吸烟是糖尿病患者周围血管病变独立危险因素(P<0.05)。调整病程、FBS、HbA1c、HDL、吸烟后rs1800592位点AA比GG患者周围血管病变患病高1.824倍(OR=1.824,P<0.05)。rs45539933、rs10011540基因多态性与周围血管病变患病风险无关(P>0.05)。病程、吸烟、rs1800592这3个交互作用模型有统计学意义(P<0.05)。结论糖尿病患者UCP1基因多态性与周围血管病变发病相关,rs1800592与吸烟、长病程的交互作用促进了糖尿病周围血管病变的发生。

Objective To investigate the relationship between UCP1 polymorphism and peripheral vascular disease in patients with diabetes mellitus.Methods From July 2019 to July 2020,440 patients with diabetes mellitus were enrolled in this study.The ankle-brachial index≤0.9 was selected as he peripheral vascular disease group,with a total of 207 patients.The ankle-brachial index was 0.9-1.4 were as the control group,with total of 233 patients.DNA was extracted from whole blood,and UCP1 gene polymorphism was detected by SNaPshot method.The relationship between UCP1 gene polymorphism and peripheral vascular disease and common risk factors were analyzed.MDR software was used to analyze the interaction between gene and environmental factors.Results There was no significant difference in BMI,SBP,DBP,TG,TC,LDL and NA between the two groups(P>0.05),while there were significant differences in the course of disease,FBS,HbA1c,HDL and smoking history between the two groups(P<0.05).Long duration of diabetes mellitus and smoking were independent risk factors for peripheral vascular disease in diabetic patients(P<0.05).After adjusting the course of disease,FBS,HbA1c,HDL,smoking rs1800592 AA was 1.824 times higher than that of GG patients with peripheral vascular disease(OR=1.824,P<0.05).The rs45539933 and rs10011540 gene polymorphisms were not associated with the risk of peripheral vascular disease(P>0.05).The three interaction models of course of disease,smoking and rs1800592 were statistically significant(P<0.05).Conclusion UCP1 gene polymorphism is associated with the incidence of peripheral vascular disease in patients with diabetic mellitus.The interaction of rs1800592 with smoking and long course of disease mellituspromotes the occurrence of diabetic peripheral vascular disease.