黄瓜多主棒孢菌致病基因CcTLS2功能探究

Studies on Function of Pathogenicity-related Gene CcTLS2 in Corynespora cassiicola of Cucumber

为探究CcTLS2基因在多主棒孢菌(Corynespora cassiicola)致病过程中的作用,以强致病力菌株HG14102524为研究对象,通过克隆多主棒孢菌CcTLS2基因,构建CcTLS2敲除株,并结合生物信息学分析、致病力测定、差异表达分析等方法,初步确定了CcTLS2对多主棒孢菌致病的影响。与野生型菌株相比,CcTLS2基因敲除菌株致病力丧失,菌落生长速度变慢,产孢量显著减少。多主棒孢菌G蛋白信号途径相关基因(A7125、A1402、A0835、A8311和A2529)在CcTLS2敲除株中的相对表达量显著下调。结果表明,CcTLS2可能通过G蛋白相关途径影响多主棒孢菌的产孢机制、菌丝生长和致病力,在多主棒孢菌致病中起重要作用。

In order to explore the effect of CcTLS2 gene in the pathogenesis of Corynespora cassiicola,this paper took the strong pathogenic strain HG14102524 as research object,constructed knockout strain by cloning the CcTLS2 gene of Corynespora cassiicola,and combined with bioinformatics analysis,pathogenicity assay,and differential expression analysis,etc.methods preliminarily determined the effect of CcTLS2 on the pathogenesis of Corynespora cassiicola.Comparing with wild type strain HG14102524,the pathogenicity of gene knockout strain CcTLS2 was lost,colony growth rate slowed down,and sporulation quantity decreased significantly.Relative expression levels of Corynespora cassiicola G protein signaling pathway related genes(A7125,A1402,A0835,A8311 and A2529)in CcTLS2 knockout strains were significantly downregulated.The results showed that CcTLS2 might pass G protein related pathway to affect the sporulation mechanism of Corynespora cassiicola,mycelia growth and pathogenicity,and played an important role in pathogenicity of C.cassiicola.