摘要
为了构建四川省2021年水稻区试品种指纹图谱,建立一套快速鉴定杂交稻种子纯度的方法,本研究结合SSR分子标记检测技术、品种指纹图谱数据并利用单粒法DNA提取和毛细管电泳系统,完成了2021年四川省水稻区试品种的指纹图谱构建和遗传多样性分析,并快速获得杂交稻惠和优566的种子纯度鉴定结果。结果表明水稻品种的指纹图谱、单粒种子提取DNA和毛细管电泳能够帮助育种者加快鉴定种子纯度。
To construct the fingerprint of rice varieties tested in the regional trials of Sichuan Province in 2021 and establish a method for rapid identification of hybrid rice seed purity,this study combined SSR molecular marker detection technology,variety fingerprint data,single seed DNA extraction and capillary electrophoresis system and completed the fingerprint construction and genetic diversity analysis of the rice varieties tested in the regional trials of Sichuan Province in 2021.The results of seed purity identification of hybrid rice Huiheyou 566 were obtained quickly.The results showed that fingerprinting of rice varieties,DNA extraction from single seeds and capillary electrophoresis could help breeders to identify seed purity faster.
作者
康亮珠
李飞
陈薇兰
王玉平
李仕贵
袁华
KANG Liangzhu;LI Fei;CHEN Weilan;WANG Yuping;LI Shigui;YUAN Hua(State Key Laboratory of Crop Gene Exploration and Utilization in Southwest/Rice Research Institute,Sichuan Agricultural University,Chengdu,Sichuan 611130,China)
出处
《杂交水稻》
CSCD
北大核心
2024年第2期29-37,共9页
Hybrid Rice
基金
四川省科技计划重点研发项目(2020YFN0146)
四川省自然科学基金面上项目(2022NSFSC0154)。
关键词
水稻品种
指纹图谱
单粒种子
DNA提取
毛细管电泳
种子纯度
快速鉴定
rice variety
fingerprint
single seed
DNA extraction
capillary electrophoresis
seed purity
rapid identification
