麻疯树提取物活性物质及抗菌作用研究

Study on the active substances and antibacterial effects of extracts from Jatropha curcas L.

使用乙醇—乙酸(1∶1)回流提取方法,获得麻疯树枝叶中的香豆素类化合物,并使用液相色谱—质谱法(LC-MS)对提取物进行定性及定量分析,通过滤纸片法探究了麻疯树中的香豆素类化合物对两种细菌的体外抑菌效果,并通过肉汤微量稀释法和生长曲线法测定麻疯树提取物(Jatropha curcas Extraction,JE)对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果表明:提取的香豆素类化合物中,主要成分为佛手柑内酯(Bergapten),相对含量为18.66%,在麻疯树化学成分研究中首次发现;同时还发现黄芩素(Baicalein)和野漆树苷(Rhoifolin),相对含量为2.76%和0.16%;麻疯树香豆素类化合物对金黄色葡萄球菌和大肠杆菌均有明显抑制效果;JE对金黄色葡萄球菌和大肠杆菌的MIC分别为1.56 mg/mL和3.125 mg/mL,对二者的MBC均为6.25mg/mL。

In this study,ethanol-acetic acid(1∶1)reflux technique was conducted to obtain J.curcas extract JE coumarins from leaves.Then,component quantification identification was measured via Liquid chromatography-mass spectrometry(LC-MS).The antibacterial function of coumarin compounds was verified by filter paper technique.Minimum Inhibitory Concentration(MIC)and Minimum Bactericidal Concentration(MBC)were measured via Broth microdilution and growth curve verification.As a result,the main component was Bergapten with 18.66%,which was firstly found in J.curcas extracts.Meanwhile,Baicalein(2.76%)and Rhoifolin(0.16%)were also discovered in the leaves extract.Antibacterial experiment resulted that coumarin could significantly suppress the growth of Staphylococcus aureus(S.anreus)and Escherichia coli(E.coli).The MIC of Jatropha curcas extracts to S.anreus and E.coli are 1.56 mg/mL and 3.125 mg/mL whereas the MBC are both 6.25mg/mL.