RNA结合蛋白RBPMS调控急性髓系白血病细胞系THP-1增殖与迁移

RNA-binding protein RBPMS regulates proliferation and migration of acute myeloid leukemia cells line THP-1

目的研究具有多重剪接功能的RNA结合蛋白(RBPMS)对携带MLL-AF9融合基因的急性髓系白血病细胞系THP-1功能的影响。方法使用GEO数据库分析造血系统中RBPMS的表达情况;通过慢病毒感染THP-1细胞,RT-qPCR和Western blot检测RBPMS过表达效率;通过高通量测序检测RBPMS过表达后对THP-1细胞转录组的影响。分别用CCK-8法和流式细胞仪检测RBPMS过表达对白血病细胞增殖以及迁移能力的影响。结果RBPMS在携带MLL-AF9融合基因的急性髓系白血病干细胞中异常低表达。RBPMS过表达后THP-1细胞的增殖和迁移能力显著降低(P<0.0001,P<0.05)。结论RBPMS可以抑制携带MLL-AF9融合基因的急性髓系白血病细胞系的细胞增殖与迁移。

Objective To investigate the impact of the RNA-binding protein with multiple splicing(RBPMS),which has multiple splicing functions,on the function of acute myeloid leukemia cells line THP-1 carrying the MLL-AF9 fusion gene.Methods The expression of RBPMS in the hematopoietic system was analyzed using the GEO database;RBPMS over-expression in THP-1 cells was achieved through lentiviral infection.The efficiency was detected by RT-qPCR and Western blot;The effect of RBPMS over-expression on the THP-1 cell transcriptome was evaluated by high-throughput sequencing.The impact of RBPMS over-expression on the proliferation and migration of leukemia cells was examined using CCK-8 assay and flow cytometry,respectively.Results RBPMS expressed in acute myeloid leukemia stem cells carrying the MLL-AF9 fusion gene was found to be inhibited.Over-expression of RBPMS significantly reduced the proliferation and migration of THP-1 cells.Conclusions RBPMS inhibits cell proliferation and migration of acute myeloid leukemia cells line carrying the MLL-AF9 fusion gene.