大蒜素抑制口腔癌细胞系CAL27的增殖

Allicin inhibits the proliferation of oral cancer cells line CAL27

目的探讨大蒜素对口腔舌鳞状细胞癌(OTSCC)细胞系CAL27增殖的影响。方法采用人OTSCC细胞系CAL27,于培养液中加入不同浓度大蒜素(5、10、20、40、60、100、200、400μmol/L)及对照DMSO处理后,进行CCK8法检测细胞增殖,RT-qPCR检测增殖相关标志物ki-67表达,以及转录本测序并进行通路富集分析。结果与对照组相比,较低浓度(20μmol/L)的大蒜素即有抑制CAL27细胞增殖的作用(P<0.01)。RT-qPCR结果显示,大蒜素能明显抑制CAL27细胞中ki-67表达,且在较低浓度(20μmol/L)就有明显抑制作用(P<0.01)。转录本测序结果显示,大蒜素能明显抑制肿瘤相关microRNA表达,尤其是miR-200a和miR-199a1的表达。结论较低浓度大蒜素即可明显抑制口腔癌细胞系CAL27的增殖,其可能通过抑制肿瘤相关microRNA来发挥作用。

Objective To explore the effect of allicin on the proliferation of oral tongue squamous cell carcinoma(OTSCC)cells line(CAL27).Methods Human CAL27 cells were used in this study.The cells were incubated with different concentrations of allicin(5,10,20,40,60,100,200,400μmol/L)and control DMSO.CCK8 assay was performed to detect cell proliferation.RT-qPCR method was used to detect the expression of proliferation related marker ki-67;Transcriptome sequencing and pathway enrichment analysis were performed.Results Lower concentrations(20μmol/L)of allicin inhibited CAL27 cells proliferation(P<0.01).Allicin significantly inhibited the expression of ki-67 in CAL27 cells at lower concentrations(20μmol/L,P<0.01).Allicin inhibited t umor related microRNA expression,especially miR-200a and miR-199a1.Conclusions Lower concentration of allicin may significantly inhibit the proliferation of oral cancer cells by inhibition of tumor related microRNAs as a potential mechanism.