蛋清蛋白絮凝处理脱除玉米秸秆水解液中的木质素衍生物

Study on the Removal of Lignin Derivatives from Corn Stalk Hydrolysate via Flocculation Treatment by Egg White Protein

为了提升玉米秸秆水解液(corn stalks hydrolysate,CSH)的可生化降解性,研究了蛋清蛋白(egg white protein,EWP)絮凝处理对CSH中木质素衍生物去除的影响,并与氯化铁、阳离子聚丙烯酰胺等常规絮凝剂的效果进行对比。结果表明,在初始CODCr浓度为40 g/L的CSH中使用EWP进行热絮凝处理(100℃下加热5 min),CSH中大分子木质素(以浊度计算)去除率达(76.87±3.00)%,显著优于氯化铁、阳离子聚丙烯酰胺的处理结果 (P <0.05)。同时,EWP热絮凝处理未造成糖、呋喃及弱酸等化合物明显损失。这是由于EWP通过疏水反应与木质素衍生物结合,进一步通过巯基-二硫键交联形成大分子化合物,促进了木质素衍生物从CSH中去除。

In order to improve the biodegradability of corn stalk hydrolysate(CSH),the effect of egg white protein(EWP)flocculation treat-ment on the removal of lignin derivatives from CSH was studied and compared with general flocculant FeCl3 and CPAM.The results showed that EWP thermal flocculation treatment(heating at 100℃for 5 min)was used in the CSH with original CODCr concentration of 40 g/L,the removal rate of macromolecular lignin(calculated by turbidity)could reach(76.87±3.00)%,which was significantly better than that of FeCl3 and CPAM(P<0.05).At the same time,EWP thermal flocculation treatment did not cause obvious loss of sugar,furan,and weak acid.Due to the sulfhydryl-disulfide bond cross-linking between EWP and lignin derivatives,macromolecular compounds formed via hydrophobic reac-tion,which promoted the removal of lignin derivatives from CSH.