黄精多糖对X射线辐射损伤小鼠外周血象及肠道菌群的影响研究

Effects of Polygonatum sibiricum Polysaccharides on Peripheral Hemogram and Gut Microbiota in Mice Irradiated by X-ray

目的探讨黄精多糖对X射线全身照射小鼠造血系统损伤和肠道菌群的作用。方法30只C57BL/6J小鼠随机分成正常对照组、照射对照组和黄精多糖给药组。分析照射前和照射后14 d内小鼠外周血白细胞、血小板和红细胞计数和不同分类白细胞百分比变化;16S rRNA高通量测序分析照后7 d小鼠肠道菌群组成和结构。结果黄精多糖组外周血白细胞数在照射后第4、7、10天,红细胞数在第1、14天均明显高于照射对照组(P<0.05),黄精多糖增加了照射后小鼠淋巴细胞百分比,降低了中性粒细胞和单核细胞的百分比。各组小鼠肠道菌群α多样性无明显改变。β多样性分析显示肠道菌群结构发生改变。功能预测分析显示,黄精多糖组参与低聚木糖、核黄素、吲哚-3-甲醛等11种物质代谢的微生物丰度明显高于正常组和照射对照组。结论黄精多糖能有效保护辐射引起的造血损伤,改变辐射所致小鼠肠道菌群结构组成和功能,为开发新型天然产物辐射防护剂提供理论依据。

Objective To investigate the radioprotective potential of Polygonatum sibiricum polysaccharides(PSP)against hemopoiesis injury and gut microbiome by X-ray.Methods Thirty C57BL/6J mice were randomly divided into three groups:normal group,irradiation group and PSP group.The white blood cell(WBC),red blood cell(RBC),platelet and the percentage of blood cells were analysed.The feces in the mouse intestines were collected at Days 7 post-radiation,and the 16S rRNA high-through put sequencing was used to detect alterations in the diversity and composition of gut microbiota.Results The counts of WBC at 4th,7th,1Oth days,and RBC at Ist,14th days after irradiation in PSP group were significantly higher than those in rradiation group(P<0.05).PSP alleviated the irradiation-induced reduction of lymphocyte percentage and attenuated the increment of neutrophil percentage and monocyte percentage after irradiation.αdiversity showed no significant differences in both irradiation group and PSP group.βdiversity showed a significant shift of microbial composition in irradiated group and PSP group.PSP treatment increased the abundance of bacteria involved in material metabolism.Conclusion PSP shows obvious radio protective effects on haemopoiesis injury and alters the gut microbiota of irradiated mice,which provide a basis for developing novel radio protective agents.