大豆C_(2)H_(2)型锌指蛋白转录因子家族全基因组鉴定及表达分析

Genome-wide Identification and Expression Analysis of C 2H 2 Zinc Finger Protein Gene Family in Soybean

为进一步寻找与大豆产量相关的潜在候选基因,利用生物信息学与转录组学技术,通过拟南芥(Arabidopsis thaliana L.)C_(2)H_(2)型锌指蛋白(C_(2)H_(2)zinc finger protein)序列BLAST映射,将从大豆(Glycine max)的基因组数据库中筛选出的44个C_(2)H_(2)锌指蛋白家族成员分成11个亚家族,并对其进行系统进化、保守结构域、顺式作用元件、测序数据和转录组数据等相关分析。结果表明:C_(2)H_(2)锌指蛋白家族成员分布于17条大豆染色体中,且每个亚家族的基因结构域较为相近,呈高度保守性。成员启动子部分含有抗逆境胁迫以及激素相关的调控元件。RNA-seq结果显示,有8个基因在不同植物组织内表达差异显著。荧光定量PCR分析表明,3个基因的分析结果为SUPERMAN的转录调节因子,GmC1-1iZFP42为Ln位点的相应基因,参与大豆叶形和籽粒的调控,GmC1-1iZFP12、GmC1-1iZFP30为SUPERMAN基因的转录调节因子,GmC1-1iZFP21可能参与大豆籽粒形成和发育的过程,为调控相关过程的候选基因。本研究为进一步寻找和挖掘大豆高产基因提供方向,也为C_(2)H_(2)型锌指蛋白和生物信息学在植物中的利用提供参考和启示。

In order to further search for potential candidate genes related to soybean yield,44 C_(2)H_(2)zinc finger protein family members from the genome database of soybean(Glycine max)were classified into 11 subfamilies by BLAST mapping of Arabidopsis thaliana L.C_(2)H_(2)zinc finger protein sequences using bioinformatics and transcriptomics technologies.The 44 C 2H 2 zinc finger protein family members screened from the soybean genome database were divided into 11 subfamilies,and analysed for phylogeny,conserved structural domains,cis-acting elements,sequencing data and transcriptome data.The results showed that the C 2H 2 zinc finger protein family members were distributed in 17 soybean chromosomes,and the structural domains of the genes in each subfamily were similar and highly conserved.The promoters of the members contained regulatory elements for stress and hormone resistance,and the RNA-seq results showed that eight genes were differentially expressed in different plant tissues.Quantitative real-time PCR analysis showed that three genes analysed were transcriptional regulators of the SUPERMAN,GmC1-1iZFP42 was the corresponding gene in the Ln locus,which is involved in the regulation of soybean leaf shape and seed,GmC1-1iZFP12 and GmC1-1iZFP30 were transcriptional regulators of the SUPERMAN gene,and GmC1-1iZFP21 may be involved in the process of soybean grain formation and development,and is a candidate gene for regulating related processes.This study provides directions for further searching and mining of soybean high yield genes,as well as references and insights for the use of C_(2)H_(2)-type zinc finger proteins and bioinformatics in plants.