苹果小G蛋白MdoROP的进化分类、结构及理化特性分析

Evolutionary classification,structure and physicochemical properties of apple small G protein MdoROP

[目的]获取6个MdoROP蛋白并对其基序组成、系统进化关系、蛋白结构及理化特性进行分析。[方法]取苹果叶片进行离体接菌,利用SMARTS和MEGA 11等多种生物信息学软件对其进行预测分析。[结果]基于蛋白基序的组成差异可将MdoROP蛋白归为同一类且均无特异性基座,表明以上6个motif为6个MdoROP蛋白的保守基序。MdoROP蛋白长度介于197~211 aa之间;脂肪指数介于87.24~92.56之间;蛋白分子量介于21463.75~23587.2 Da;6个MdoROP蛋白的亲水性介于-0.125~-0.077且均为负值,6个MdoROP蛋白均为碱性亲水蛋白,均定位于细胞膜、细胞质和细胞核且均不具有信号肽和跨膜结构域;MdoROP蛋白的二级结构元件以无规则卷曲和α螺旋为主。[结论]6个MdoROP蛋白属于苹果小G蛋白ROP家族,是磷酸化蛋白和碱性亲水蛋白,均不是分泌蛋白和膜蛋白;无规则卷曲和α螺旋是6个MdoROP蛋白的主要二级结构,ROP基因三级结构的预测结果与二级构造相吻合。该文研究结果将为后续深入研究MdoROP蛋白的生物学功能奠定强有力的基础并提供理论支撑。

[Objective]To obtain six MdoROP proteins and analyze their motif composition,phylogenetic relationship,protein structure and physicochemical properties.[Method]Apple leaves were taken for ex vivo sterilization,and various bioinformatics software such as SMARTS and MEGA 11 were used to predict and analyze them.[Result]The MdoROP proteins can be categorized into the same class and all have no specific motifs based on differences in the composition of the protein motifs.It is shown that the above 6 motifs are the conserved motifs of 6 MdoROP proteins.The length of MdoROP protein was between 197-211 aa;The fat index is between 87.24-92.56;The molecular weight of protein is 21463.75-23587.2 Da;The hydrophilicity of 6 MdoROP proteins was between-0.125--0.077 and all were negative,and all 6 MdoROP proteins were alkaline hydrophilic proteins,all of which were localized to cell membrane,cytoplasm and nucleus and did not have signal peptides and transmembrane domains.The secondary structural elements of MdoROP protein are mainly irregular coil andαhelix.[Conclusion]The six MdoROP proteins belong to the small G protein ROP family of apples,which are phosphorylated proteins and basic hydrophilic proteins,neither of which are secreted proteins or membrane proteins.Irregular coils andαhelixes were the main secondary structures of the six MdoROP proteins,and the prediction results of the tertiary structure of ROP genes were consistent with the secondary structure.In summary,the results of this study will lay a strong foundation and provide theoretical support for the subsequent in-depth study of the biological function of MdoROP protein.It contributes to the subsequent in-depth study of the biological functions of apple MdoROP proteins.