摘要
斑马鱼(Danio rerio,zebra fish)是一种新型脊椎动物模型,由于具有与人类同源性高、价格低廉、生长周期短、透明度高等优点,已应用于人类疾病研究。该文分析对比了斑马鱼12个已通过测量目标mRNA或蛋白质水平证明功能丧失的ASD风险基因与人类基因的同源性百分比,从基因组靶向诱变方面介绍了9个斑马鱼功能敲低或敲除且有显著ASD行为的ASD模型,从药物环境诱导方面介绍了丙戊酸钠、戊四唑等环境因素诱导的斑马鱼ASD模型,以及对应的ASD行为表现。斑马鱼对于孤独症谱系障碍(autism spectrum disorder,ASD)的机理研究、药物治疗与筛选等研究具有独特的优势,在人类疾病的研究中将会带来极大的研究前景和应用价值。
Danio rerio(zebra fish)is a new vertebrate model.Due to its high homology with human,low price,short growth cycle and high transparency,it had been applied in the study of human diseases.This review analyzed and compared the homology percentage of 12 zebrafish ASD risk genes with functional loss demonstrated by measuring target mRNA or protein levels and human genes,and introduced 9 zebrafish ASD models with functional knockdown or knockdown and significant ASD behavior from the aspect of genomic targeted mutagenesis.From the aspect of drug environment induction,the model of zebrafish ASD induced by environmental factors such as sodium valproate and pentatetrazole and the corresponding behavior of ASD were been introduced.Zebrafish has unique advantages for research on the mechanism,drug treatment and screening of autism spectrum disorder(ASD),but there are still many research and application values that have not been explored,which will bring great research prospects and application values in the study of human diseases.
作者
陆燕琴
尚鑫淼
向往
王娟
徐新杰
张嵘
王军
LU Yanqin;SHANG Xinmiao;XIANG Wang;WANG Juan;XU Xinjie;ZHANG Rong;WANG Jun(Hubei University of Technology,Industrial Fermentation Collaborative Innovation Center Biomedical and Molecular Diagnostics Team,Wuhan 430068,China;Hubei University of Technology,Autistic Depression Diagnostic and Intervention Institute,Wuhan 430068,China;Hubei University of Technology,Cell Regulation and Molecular Drug“111”Introduction Center,Wuhan 430068,China;Department of Nephrology,East Hospital of Wuhan University People′s Hospital,Wuhan 430060,China;Peking Union Medical College Hospital,Beijing 100010,China;Peking University Health Science Center,Beijing 100191,China)
出处
《生物技术》
CAS
2024年第1期126-133,共8页
Biotechnology
基金
科技部高端外国专家项目(G2022027010L)
湖北省教育厅创新团队项目(T2020009)
湖北省高端人才项目(337/370)。
关键词
斑马鱼模型
孤独症谱系障碍
生理特性
行为
基因同源性
基因修饰
环境因素
应用进展
zebra fish model
autism spectrum disorder
physiological characteristics
behavior
gene homology
gene modification
environmental factor
current progress