单细胞RNA测序及其在肺癌诊疗中的应用进展

Advances in single-cell RNA sequencing and its applications in lung cancer

肺癌具有高度异质性,其在我国恶性肿瘤中的发病率和死亡率均居第1位。传统的RNA测序技术只能反映样品的平均基因表达水平,近年发展起来的单细胞RNA测序(scRNA-seq)技术通过识别不同的细胞亚群,探索肿瘤异质性和解剖肿瘤微环境,为理解复杂的肿瘤生物学铺平了道路。目前scRNA-seq技术在异质性、肿瘤微环境、生物标志物、治疗和耐药性等方面越来越多地应用于肺癌的研究。文章综述了scRNA-seq技术的基本发展过程及其在肺癌研究领域的应用现状。

Lung cancer is highly heterogeneous and ranks the first in cancer incidence and mortality in China.Previous bulk RNA sequencing techniques can only reflect the average gene expression of the samples.The single-cell RNA sequencing(scRNA-seq)technology developed in recent years have paved the way for understanding complicated tumor biology by identifying different cell subpopulations,exploring tumor heterogeneity,and dissecting the tumor microenvironment.scRNA-seq technology has been increasingly used in lung cancer research to explore lung cancer from its heterogeneity,tumor microenvironment,biomarkers,therapy,and drug resistance.This review summarizes the basic processes and development of scRNA-seq techniques and their applications in the field of lung cancer research.