人巨细胞病毒微小RNA UL112调控血管内皮细胞mRNA的研究

Study of human cytomegalovirus microRNA UL112 regulating vascular endothelial cell mRNA

目的探讨人巨细胞病毒RNA UL112对人血管内皮细胞mRNA表达的影响。方法构建人巨细胞病毒微小RNA UL112腺病毒过表达载体并使其感染人原代脐静脉内皮细胞作为转染组,感染无义空白腺病毒载体的脐静脉内皮细胞为对照组。检测人巨细胞病毒微小RNA UL112对脐静脉内皮细胞mRNA表达谱的影响。结果与对照组相比,转染组mRNA存在差异表达基因365个,其中表达基因上调303个,下调62个,差异表达基因富集于丝裂原活化蛋白激酶通路。结论人巨细胞病毒微小RNA UL112影响脐静脉内皮细胞mRNA的表达。

Objective To investigate the effect of human cytomegalovirus microRNA UL112(HCMV miR-UL112)on human vascular endothelial.Methods A HCMV miR-UL112 adenovirus overexpression vector was constructed and used to infect human primary umbilical vein endothelial cells(HUVECs)as the transfection group,while HUVECs infected with an ineffective adenovirus served as the control group.The effect of HCMV miR-UL112 on mRNA expression profile of HUVECs were detected.Results In the transfection group,there were 365 differentially expressed genes compared to the control group,with 303 upregulated genes and 62 downregulated genes.The differentially expressed genes were enriched in the mitogen-activated protein kinase(MAPK)signaling pathway.Conclusion HCMV miR-UL112 can affect mRNA expression of HUVECs.