粉葛再生体系建立的研究

THE ESTABLISHMENT OF THE REGENERATION SYSTEM OF PUERARIA THOMSONII BENTH

为建立高效粉葛组织培养再生体系,以不同消毒方式、基本培养基、蔗糖浓度、外植体等处理,研究其对粉葛初代培养、继代培养和生根培养的影响。结果表明,以75%酒精30 s+0.1%升汞+吐温-80消毒8 min时,外植体的成活率最高,可达83.33%。外植体的最佳取材部位为带芽茎段,其平均株高可达2.13 cm,繁殖系数为2。MS为最佳的基本培养基,繁殖系数为1.26;蔗糖浓度以30 g/L为最佳,其平均株高为1.69 cm,繁殖系数为1.55;最佳初代培养基为MS+琼脂5.5 g/L+蔗糖30 g/L+6-BA 0.2 mg/L+IBA 0.6 mg/L,其平均株高为3.13 cm,繁殖系数为3.17;最佳的继代培养基为MS+琼脂5.5 g/L+蔗糖30 g/L+6-BA 0.6 mg/L+IBA 0.8 mg/L+KT 0.2 mg/L,其平均株高为1.86 cm,繁殖系数为3.44;最佳的生根培养基为MS+琼脂5.5 g/L+蔗糖30 g/L+IBA 1.0 mg/L,其生根率为100%,平均主根数达7.1根。通过组培条件优化,初步建立粉葛的再生体系。

In order to establish an efficient tissue culture regeneration system of Pueraria thomsonii Benth,the effects of different disinfection methods,basic medium,sucrose concentration and explants on the primary culture,secondary culture and rooting culture of P.thomsonii were studied.The results showed that the survival rate of explants was the highest when disinfected with 75%alcohol for 30 s+0.1%mercury+Tween-80 for 8 min,reaching 83.33%.The best part of the explants was the stem segment with buds,the average plant height could reach 2.13 cm,and the reproduction coefficient was 2.MS was the best basic medium with a reproductive coefficient of 1.26,sucrose concentration was the best,the average plant height was 1.69 cm,the reproductive coefficient was 1.55,the optimal primary medium was MS+agar 5.5 g/L+sucrose 30 g/L+6-BA0.2 mg/L+IBA0.6 mg/L,the average plant height was 3.13 cm,the reproductive coefficient was 3.17,and the optimal submedium was MS+agar 5.5g/L+sucrose 30 g/L+6-BA0.6 mg/L+IBA0.8 mg/L+KT0.2 mg/L,the average plant height was 1.86 cm,the reproduction coefficient was 3.44,and the optimal rooting medium was MS+agar 5.5 g/L+sucrose 30g/L+IBA1.0 mg/L,the rooting rate was 100%,and the average number of taproots was 7.1.The regeneration system of P.thomsonii was established by optimizing tissue culture conditions.