基于金纳米复合物的对碱性橙21可视化快速分析检测

A Rapid Detection Method Forpigment Based on Ps-Co-P4VP/AU Peroxidase Activity

以苯乙烯和4-乙烯基吡啶为单体,采用乳液聚合法一步合成了聚苯乙烯-聚4-乙烯基吡啶核壳型微球。将其与金纳米粒子结合后形成具有过氧化物酶活性的聚苯乙烯-聚4-乙烯基吡啶/金(PS-co-P4VP/Au)复合纳米材料。该微球粒径均一,形貌良好,在有过氧化氢(H_(2)O_(2))存在的情况下,能催化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色产物。该材料在pH=9时对碱性橙21具有良好的吸附性能,且吸附会降低其过氧化物酶活性,降低值与色素浓度呈线性关系。据此建立了一种快速检测碱性橙21的方法,最佳检测条件为:pH=9,TMB浓度1.4 mmol/L,H_(2)O_(2)浓度140 mmol/L,材料浓度2.5 mg/mL。该条件下碱性橙21的检出限为0.6μg/mL,在配制酒和水果糖中的回收率为92.2%~102.8%。

Polystyrene-poly(4-vinylpyridine)core-shell microspheres were synthesized by lotion polymerization with styrene and 4-vinylpyridine as monomer.It was combined with gold nanoparticles to form polystyrene-poly(4-vinylpyridine)/gold(PS-co-P4VP/Au)composite nanosphere with spherical morphology,favorable dispersity.In the presence of hydrogen peroxide,3,3′,5,5′-tetramethylbenzidine(TMB)can be catalyzed by the microspheres with peroxidase activity to generate blue products.The optimum pH of the material for adsorbing basic orange 21 was 9.It was found that the peroxidase activity of microspheres will be decreased after adsorption,and the decrease is linear with the pigment concentration,based on the phenomenon,a rapid detection method for basic orange 21 was established.When the pH=9,TMB concentration was 1.4 mmol/L,H_(2)O_(2)concentration was 140 mmol/L,and material concentration was 2.5 mg/ml,the detection effect is the best.And under this conditions,the detection limit of basic orange 21 was 0.6μg/mL,the recovery rate in the wine and sugar was 92.2%~102.8%.