摘要
以苯乙烯和4-乙烯基吡啶为单体,采用乳液聚合法一步合成了聚苯乙烯-聚4-乙烯基吡啶核壳型微球。将其与金纳米粒子结合后形成具有过氧化物酶活性的聚苯乙烯-聚4-乙烯基吡啶/金(PS-co-P4VP/Au)复合纳米材料。该微球粒径均一,形貌良好,在有过氧化氢(H_(2)O_(2))存在的情况下,能催化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色产物。该材料在pH=9时对碱性橙21具有良好的吸附性能,且吸附会降低其过氧化物酶活性,降低值与色素浓度呈线性关系。据此建立了一种快速检测碱性橙21的方法,最佳检测条件为:pH=9,TMB浓度1.4 mmol/L,H_(2)O_(2)浓度140 mmol/L,材料浓度2.5 mg/mL。该条件下碱性橙21的检出限为0.6μg/mL,在配制酒和水果糖中的回收率为92.2%~102.8%。
Polystyrene-poly(4-vinylpyridine)core-shell microspheres were synthesized by lotion polymerization with styrene and 4-vinylpyridine as monomer.It was combined with gold nanoparticles to form polystyrene-poly(4-vinylpyridine)/gold(PS-co-P4VP/Au)composite nanosphere with spherical morphology,favorable dispersity.In the presence of hydrogen peroxide,3,3′,5,5′-tetramethylbenzidine(TMB)can be catalyzed by the microspheres with peroxidase activity to generate blue products.The optimum pH of the material for adsorbing basic orange 21 was 9.It was found that the peroxidase activity of microspheres will be decreased after adsorption,and the decrease is linear with the pigment concentration,based on the phenomenon,a rapid detection method for basic orange 21 was established.When the pH=9,TMB concentration was 1.4 mmol/L,H_(2)O_(2)concentration was 140 mmol/L,and material concentration was 2.5 mg/ml,the detection effect is the best.And under this conditions,the detection limit of basic orange 21 was 0.6μg/mL,the recovery rate in the wine and sugar was 92.2%~102.8%.
作者
田甜
张毅
严恒
谢卫红
TIAN Tian;ZHANG Yi;YAN Heng;XIE Weihong(School of Biological Engineering and Food,Hubei Univ.of Tech.,Wuhan 430068,China;Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075,China)
出处
《湖北工业大学学报》
2024年第2期93-97,110,共6页
Journal of Hubei University of Technology
基金
湖北省食品质量安全监督检验研究院科技项目(ZZLX2015015)
湖北工业大学“产品质量工程”跨学科研究院开放课题(00021)。
关键词
金纳米颗粒
复合材料
碱性橙21
色素检测
gold nanoparticles
composites
alkaline orange 21
pigment detection