产志贺毒素大肠埃希氏菌活菌检测方法的应用现状及展望

Application Status and Prospects of Detection Methods for Living Shiga toxin-producing Escherichia coli

产志贺毒素大肠埃希氏菌作为一种重要的食源性致病菌,会对人体健康造成严重威胁。对其进行快速、准确的鉴定检测是预防和控制相关疾病的有效手段。基于此,文章对目前可用于检测活体产志贺毒素大肠埃希氏菌的方法进行综述,主要包括传统的细胞培养方法、免疫学检测方法和分子生物学方法等,并对这些方法的应用和发展前景展开了深入探讨。随着分子生物学和生物工程技术的发展,不同检测方法联合使用可以克服单一方法的缺陷,从而取得更高效、准确的检测结果。

Shiga toxin-producing Escherichia coli(STEC),as an important foodborne pathogenic bacteria,is a serious threat to human health.The rapid and accurate detection of STEC is an effective method for the prevention and control of relative diseases.This paper reviews the current methods used to detect living STEC,which mainly include traditional cell culture method,immunological detection method and molecular biology method,and discusses the application and development prospects of these methods.With the development of molecular biology and bioengineering technology,the joint use of different detection methods can overcome the defects of using a single method,and achieve more efficient and accurate detection objectives.