杜仲类钙调蛋白EuCML5基因功能分析

Functional Analysis of Eucommia ulmoides Calmodulin EuCML5 Gene

文章通过构建植物表达载体pSH737-35S-Eu CML5,将目的基因转入模式植物烟草,获得转基因植株,并对转EuCML5基因、转空载体和野生型烟草植株同时进行低温(4℃)、高温(42℃)、干旱和高盐四种非生物胁迫处理,以观察烟草植株的表型变化。结果发现,在四种非生物胁迫处理下,转EuCML5基因烟草的存活率均高于野生型和转空载体烟草的存活率;同时,转EuCML5基因烟草的叶片卷曲率均低于野生型和转空载体烟草。可见,过表达Eu CML5能增强烟草对低温(4℃)、高温(42℃)、干旱和高盐环境的耐受性。这将为进一步了解植物耐逆分子机制以及利用该基因进行作物抗逆性改良奠定基础。

This study constructed a plant expression vector pSH737-35S-EuCML5,and transferred the target gene into the model plant tobacco to obtain transgenic plants.The transgenic EuCML5 gene,empty vector,and wild-type tobacco plants were subjected to four abiotic stress treatments simultaneously,including low temperature(4℃),high temperature(42℃),drought,and high salt,in order to observe the phenotypic changes of tobacco plants.The results showed that under four types of abiotic stress treatments,the survival rates of EuCML5 transgenic tobacco were higher than that of wild-type and empty vector tobacco,and the leaf curling rate of EuCML5 transgenic tobacco was lower than that of wild-type and empty vector tobacco.Overexpression of EuCML5 can enhance tobacco's tolerance to low temperature(4℃),high temperature(42℃),drought,and high salt,which will lay the foundation for further understanding the molecular mechanisms of plant stress tolerance and utilizing this gene for crop stress improvement.