海茴香植物乳杆菌发酵物对黑色素生成抑制作用及安全性评估

Inhibitory Effect and Safety Evaluation of Crithmum Maritimum Fermentation

通过发酵技术获得海茴香植物乳杆菌发酵物(CMF),利用酪氨酸酶、B16黑素瘤细胞、人皮肤角质形成细胞(HaCaT)作为测试模型,考察CMF对黑色素生成相关靶点的作用,并考察其使用安全性。结果证实CMF具有较强的自由基清除能力,其IC50值为0.87%,该发酵物可有效抑制细胞内酪氨酸酶活性,并能显著抑制小眼畸形转录因子(MITF)mRNA以及酪氨酸酶相关蛋白-1(TRP-1)mRNA的表达。质量分数2.0%的CMF可使B16黑色素瘤细胞中黑色素生成率降低至33.82%。CMF还对人肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的生成产生抑制作用。通过红细胞溶血试验以及人体斑贴测试证实,CMF对皮肤无局部刺激性。

Crithmum maritimum fermentation product(CMF)was obtained by fermentation technology.The effects of CMF on melanogenesis and melanogenesis-related targets were investigated using tyrosinase,B16 melanoma cells and human immortal keratinocyte line cells(HaCaT)as experimental models.The safety experiments were also carried out to detect the satety of its application.The results claimed that CMF possessed significantly high DPPH radical scavenging activities with IC50 value of 0.87%.It could not only effectively inhibit the activity of tyrosinase in cells,but also significantly inhibit the relative expression of microphthalmia-associated transcription factor(MITF)mRNA and tyrosinase-related protein 1(TRP-1)mRNA.Only 33.82%residual tyrosinase activity was retained in B16 cells in contrasting with the model group with the addition of 2%(in mass fraction)CMF.In addition,CMF had an effect on decreasing the production of tumor necrosis factor-α(TNF-α)and interleukin(IL-1β)which played an vital importance in the activation of the tyrosinase.The red blood cell hemolysis test and human patch test confirmed that the CMF had no biological toxicity and could be used in cosmetics safely.