张家口种植紫苏叶中总挥发油成分的提取及含量测定

Extraction and Content Determination of Total Volatile Oil in Perilla Leaf Grown in Zhangjiakou

目的:用高效液相色谱法分别测定市场销售的紫苏叶和河北北方学院百草园种植的紫苏叶中紫苏醛的含量,并计算总挥发油含量,通过比较总挥发油的含量评价我校百草园种植的紫苏叶是否达到市售标准。方法:用加热回流法,20倍无水乙醇,于85℃水浴中加热回流1 h,分别提取两种紫苏叶中总挥发油成分。以紫苏醛为对照品,采用HPLC法测定总挥发油中紫苏醛的含量,计算得出两组紫苏叶中总挥发油的含量。结果:由紫苏醛对照品的线性关系回归方程计算得出,市场销售的紫苏叶中紫苏醛的含量为0.109%,总挥发油的含量为1.162%,河北北方学院种植的紫苏叶中紫苏醛的含量为0.102%,总挥发油的含量为1.090%。结论:河北北方学院百草园种植紫苏叶的挥发油含量虽然略低于市场销售的紫苏叶,但也已经达到了市场销售的标准。

Objective:The contents of total volatile oil in Perilla leaves sold on the market and those planted in Baicao Garden of Hebei North University were determined by HPLC.The contents of total volatile oil were compared to evaluate whether the Perilla leaves planted in Baicao Garden of our school reached the market standard.Methods:Total volatile oils were extracted from two kinds of Perilla leaves by heating reflux method with 20 times anhydrous ethanol in 85℃water bath for 1h.The content of Perilla aldehyde in total volatile oil was determined by HPLC,and the content of total volatile oil in two groups of Perilla leaves was calculated.Results:According to the linear regression equation,the content of Perilla aldehyde and total volatile oil in Perilla leaves sold on the market was 0.109%and 1.162%.The content of total volatile oil in Perilla leaves planted in our school was 0.102%and 1.090%.Conclusion:Although the content of volatile oil of Perilla leaves planted in Baicao Garden of our school is slightly lower than that of Perilla leaves sold on the market,it has reached the standard of market sale.