摘要
通过索式提取法获得了八角茴香粉末的无水乙醇提取液,测定了提取液对褐飞虱的驱避、触杀、熏蒸活性等影响。结果表明,与对照水稻相比,褐飞虱在八角茴香提取液处理的水稻上取食和产卵都极显著减少(P<0.01),产卵量分别仅为对照水稻的36.11%,产卵忌避率达46.94%;而48 h内拒食率为35.38%~66.78%。八角茴香提取液对褐飞虱若虫具一定的熏蒸活性,24 h和48 h平均校正死亡率分别为9.62%和13.98%,两者间无显著差异。几率值法测定八角茴香提取液对褐飞虱若虫触杀活性较好,致死中浓度LC_(50)分别为7.04和4.69 mg·mL^(-1)。说明八角茴香中存在对褐飞虱行为调控和杀虫活性物质,具有作为植物源杀虫剂用于田间防控褐飞虱的潜在价值。
The extracts of absolute ethyl alcohol from star anise(Illicium verum)powder were obtained by soxhlet extractor method,and effects of the extracts on repellency,contact toxicity and fumigation activity of brown planthopper(BPH)were detected.The results showed that the number of BPH chose to feed and lay eggs on rice treated with extracts of star anise decreased significantly compared with control rice(P<0.01),and the number of eggs on star anise extracts treated rice plants was only 36.11%of that of control rice,oviposition deterrent rate reach to 46.94%;the antifeeding rate ranged from 35.38%-66.78%in 48 h.Extracts of star anise showed not very strong fumigation activity to BPH,the mean corrected mortality rates at 24 h and 48 h were 9.62%and 13.98%,respectively.Probability value method was used to determine the contact toxicity of star anise extracts against brown planthopper nymphs,the half lethal concentrations(LC 50)were 7.04 and 4.69 mg·mL^(-1).It was showed that there are some active substances in star anise for behavior regulation and insecticidal activity of BPH,which has potential value for the prevention and control of BPH in field.
作者
沈炜
曹梦娇
王霞
SHEN Wei;CAO Mengjiao;WANG Xia(Agriculture Technology Service Center of Qixing Street,Nanhu District,Jiaxing 314002,Zhejiang;Jiaxing Soil and Fertilizer,Plant Protection and Rural Energy Station,Jiaxing 314050,Zhejiang;College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,Zhejiang)
出处
《浙江农业科学》
2024年第4期962-966,共5页
Journal of Zhejiang Agricultural Sciences
基金
浙江省“三农六方”项目(2022SNJF011、CTZB-F170623LWZ-SNY1-9)
国家自然科学基金(32072508)
宁波市重大科技任务攻关项目(2022Z182)。
关键词
八角茴香
褐飞虱
生物活性
索式提取法
Illicium verum
brown planthopper
biological activity
soxhlet extraction method