尖孢镰刀菌对秀丽隐杆线虫生物学特性及表达转录组的影响

Effects of Fusarium oxysporum on the biological characteristics and expression transcriptome of Caenorhabditis elegans

【目的】探讨尖孢镰刀菌Fusarium oxvsporum对秀丽隐杆线虫Caenorhabditis elegans生物学特征的影响,明确线虫响应尖孢镰刀菌侵染的关键通路。【方法】将尖孢镰刀菌活性孢子和灭活孢子分别与秀丽隐杆线虫共培养,测定C.elegans寿命、体长、运动能力、繁殖力以及咽泵运动速率等基本生物学特征;进行全基因组测序,通过GO和KEGG分析明确线虫响应尖孢镰刀菌侵染的关键通路和生物学功能。【结果】尖孢镰刀菌活性分生孢子与线虫共培养可导致线虫寿命下降,虫体显著缩短,线虫繁殖力显著降低;但共培养并未对线虫的咽泵运动速率及运动能力产生明显影响。GO分析发现,差异表达基因富集在先天免疫反应和氧化还原酶活性等生物学功能方面;KEGG分析发现,差异表达基因富集在药物代谢−细胞色素P450、代谢外源性物质的细胞色素P450和脂肪酸代谢等通路。【结论】尖孢镰刀菌对秀丽隐杆线虫的基本生命活动有不利影响,这为深入探讨线虫响应尖孢镰刀菌的天然免疫机制及与植物免疫的相似性,从而寻找植物相关抗病基因奠定了基础。

【Objective】To investigate how Fusarium oxysporum affects the fundamental biological traits of nematodes(Caenorhabditis elegans),and identify the critical mechanism of nematode responding to F.oxysporum infection.【Method】The active and inactive spores were cocultured with C.elegans to determine the biological characteristics of C.elegans,such as lifespan,body length,motility/reproductive ability and pharyngeal pumping rate.The whole genome sequencing was performed to find the key pathways and biological functions of nematode responding to F.oxysporum infection by GO and KEGG analysis.【Result】The coculture with active spores shortened the longevity of nematodes,significantly decreased the body size and reduced the reproductive ability of nematodes.However,the coculture did not obviously affect the pharyngeal pumping rate and motility ability of nematodes.GO analysis showed that the differentially expressed genes were enriched in biological functions such as innate immune response and oxidoreductase activity.KEGG analysis showed that the differentially expressed genes were enriched in cytochrome P450 metabolizing drug,cytochrome P450 metabolizing xenobiotics,fatty acid metabolism and other pathways.【Conclusion】F.oxysporum has adverse effects on the basic life activities of C.elegans,which provides insight into the natural immune mechanism of nematodes in response to F.oxysporum and the similarity with plant immunity,so as to find plant-related disease resistance genes.