β-肌动蛋白、高迁移率蛋白A2在子宫内膜腺癌组织中的表达及与预后的关系

Expressions of β-actin and high mobility protein A2 in the endometrial adenocarcinoma tissues of patients and their correlation with the prognosis of the patients

目的:探讨β肌动蛋白(ACTB)、高迁移率蛋白A2(HMGA2)在子宫内膜腺癌组织中的表达及与预后的关系.方法:选择2017年10月-2020年10月河北省唐山市妇幼保健院收治的206例子宫内膜腺癌患者,取手术切除的癌组织和癌旁组织,实时荧光定量聚合酶连反应检测子宫内膜腺癌组织和癌旁组织中ACTB、HMGA2表达,比较不同病理特征子宫内膜腺癌组织的ACTB、HMGA2表达差异,术后定期电话随访至2022年10月,分析ACTB、HMG GA2表达与预后的关系.结果:子宫内膜腺癌组织ACTBmRNA及HMGA2mRNA表达水平分别高于其相应癌旁组织(3.65±1.09比1.12±0.36,P<0.05)及(5.03±1.46比1.65±0.47)差异有统计学意义(P<0.05),TNM分期Ⅲ期、深层浸润、盆腔淋巴结转移患者的子宫内膜癌组织中ACTBmRNA、HMGA2mRNA表达高于TNM分期Ⅰ~Ⅱ期、无盆腔淋巴结转移宫颈癌患者(P<0.05).中位随访48(24~60)个月,随访期间死亡62例,ACTBmRNA、HMG GA2mRNA高表达的子宫内膜腺癌患者总生存率为59.1%、61.3%,低于低表达患者的81.2%、79.0%(P<0.05).统计学分析显示盆腔淋巴结转移、ACTBmRNA高表达、HMGA2mRNA高表达是子宫内膜腺癌患者不良预后的独立危险因素(P<0.05).结论:子宫内膜腺癌组织中ACTBmRNA、HMGA2mRNA表达上调,且与子宫内膜腺癌恶性进展和总生存率低下相关.

Objective:To investigate the expressions of β-actin(ACTB)and high mobility protein A2(HMGA2)in the endometrial adenocarcinoma tissues of patients,and to study their correlation with the prognosis of the patients.Methods:A total of 206 patients with endometrial adenocarcinoma who had been treated in hospital from October 2017 to October 2020 were selected in this study.The expressions of ACTB and HMGA2 in the endometrial adenocarcinoma tissues and in the adjacent tissues of endometrial adenocarcinoma were detected by real-time fluorescent quantitative polymerase chain reaction.The expression differences of ACTBand HMGA2 in the endometrial adenocarcinoma tissues were compared among the patients with different pathological characteristics.The patients were followed up by telephone regularly after surgery until October 2022.The correlation between the expressions of ACTBand HMGA2 in the endometrial adenocarcinoma tissues of the patients and their prognosis was analyzed.Results:The expression levels of the ACTB mRNA(3.65±1.09)and the HMGA2 mRNA(5.03±1.46)in the endometrial adenocarcinoma tissues of the patients were significantly higher than those(1.12±0.36 and 1.65±0.47)in their corresponding adjacent tissues of the endometrial adenocarcinoma(P<0.05).The expressions of the ACTB mRNAand the HMGA2 mRNA in the endometrial adenocarcinoma tissues of the patients with the TNM stage Ⅲ,the deep invasion and the pelvic lymph node metastasis of the endometrial adenocarcinoma were significantly higher than those of the patients with the TNM stage Ⅰ to Ⅱ of adenocarcinoma and without pelvic lymph node metastasis(P<0.05).The median follow-up was 48(24-60)months,and there were 62 cases died during the follow-up period.The overall survival rates of the patients with the endometrial adenocarcinoma and with the high expressions of ACTB mRNAand HMGA2 mRNA were 59.1% and 61.3%,and which were significantly lower than those(81.2% and 79.0%)of the patients with endometrial adenocarcinoma and with the low expressions of ACTB mRNA and HMGA2 mRNA(P<0.05).Statistical analysis showed that the pelvic lymph node metastasis and the high expressions of ACTB mRNA and HMGA2 mRNA of the patients with the endometrial adenocarcinoma were the independent risk factors of their adverse prognosis(P<0.05).Conclusion:The expressions of ACTB mRNAand HMGA2 mRNA in the endometrial adenocarcinoma tissues of the patients are up-regulated,which are associated with the malignant progression of the endometrial adenocarcinoma and the low overall survival rate of the patients.