HIF-1α与miR-210-3p在子宫内膜异位症组织中的表达及相关性

Expression levels of hypoxia-inducible factor-1 alpha and microRNA-210-3p in endometriosis and their correlation

目的研究缺氧诱导因子1α(HIF-1α)与微小RNA-210-3p(miR-210-3p)在子宫内膜异位症(EMS)患者异位内膜组织中的表达。方法选取56例EMS手术患者,所有病例均经腹腔镜或开腹手术确诊,经病理证实为EMS,术中留取异位内膜组织为试验组,同期选取正常子宫内膜组织22例为对照组,应用免疫组织化学法(SP)测定2组内膜组织中HIF-1α的表达,并采用实时荧光定量法(qRT-PCR)检测2组标本中miR-210-3p相对表达水平;采用Spearman法分析异位内膜组织中HIF-1α与miR-210-3p表达水平相关性分析。结果异位内膜组织中miR-210-3p相对表达量显著高于正常内膜组织,差异有统计学意义(P<0.05);异位内膜组织中HIF-1α蛋白的阳性表达率高于正常内膜组织,差异有统计学意义(P=0.036);Spearman相关性分析结果显示异位内膜组织中HIF-1α与miR-210-3p表达水平呈正相关(r=0.573,P<0.001)。结论EMS患者异位内膜组织中HIF-1α与miR-210-3p表达上调,两者呈正相关,可能协同参与了EMS的发生发展。

Objective To measure the expression levels of the hypoxia-inducible factor-1 alpha(HIF-1α)and microRNA-210-3p(miR-210-3p)in the ectopic endometrial tissues of patients with ovarian endometriosis(EMs).Methods A total of 56 patients with ovarian EMs who were managed by laparoscopy or laparotomy and confirmed by pathologically were included.Ectopic endometrial tissues were intraoperatively collected as test group.During the same period,22 normal endometrial tissues were selected as control group.Positive expression of HIF-1α in endometrium tissues was determined by immunohistochemistry for surgical specimen(SP).The quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the relative expression of miR-210-3p.Spearman correlation was used to analyze the correlation between expression levels of HIF-1α and miR-210-3p in the ectopic endometrial tissues of patients with ovarian EMs.Results The relative expression of miR-210-3p in the ectopic endometrial tissues was significantly higher than that of normal endometrium tissues(P<0.05).The positive expression rate of HIF-1α protein in the ectopic endometrial tissues was significantly higher than that of normal endometrium tissues(P=0.036).Spearman correlation analysis showed that there was a positive correlation between the expression levels of HIF-1α and miR-210-3p in the ectopic endometrial tissues of patients with ovarian EMs(r=0.573,P<0.001).Conclusion HIF-1α and miR-210-3p are overexpressed in the ectopic endometrial tissues of patients with ovarian EMs and positively correlated,which may be synergistically involved in the occurrence and progression of ovarian EMs.