基于数据挖掘的角膜新生血管动物模型应用特点分析

Application of Animal M odels of Corneal Neovascularization Based on Data Mining

目的:通过总结角膜新生血管(CNV)动物模型的造模特点,为建立理想的CNV动物模型提供参考依据。方法:该文以“角膜新生血管”为主题词在中国知网、万方数据、中华医学期刊全文数据库及PubMed数据库进行检索,筛选出2013—2023年中有关CNV动物实验的相关文献,通过Excel 2021表格建立数据库,对其实验动物品系、性别、造模方法、检测指标、应用类别等内容进行整理,分析CNV动物模型的模型特点。结果:经过对比分析发现造模时的动物品系以SD大鼠(87次、29.49%),新西兰白兔(52次、17.63%)为主;动物性别推荐单一性别,目前以雄性为主;药效验证和机制研究的造模方法均以碱烧伤法居多;指标检测方式包括表观指标观察、组织病理学检测、免疫组化(IHC)、蛋白免疫印迹法(Western blot)及多种聚合酶链式反应(PCR)检测为主;检测指标包括表观指征、角膜组织病理、CNV调控相关指标等。结论:模型复制推荐碱烧伤法诱导的SD大鼠CNV模型,指标主要选择CNV的生长情况、角膜组织病理检测及血管内皮生长因子(VEGF)相关检测,依照需求增选角膜表观指征及血管内皮细胞生长因子受体2(VEGFR2)、碱性成纤维细胞生长因子(bFGF)、分泌粒蛋白Ⅲ(Scg3)等CNV调控相关基础指标。临床治疗CNV用药以抗炎药物与抗VEGF药物为主,缺乏中医药应用,模型的完善需要增加中医证候要素。

Objective:To provide a reference for the establishment of an ideal corneal neovascularization(CNV)animal model by summarizing the modeling characteristics of CNV animal models.Method:With"CVN"as the theme word,this paper searched the China National Knowledge Infrastructure(CNKI),Wanfang,Chinese medical journals full-text database,and PubMed database and screened out relevant literature on CNV animal experiments from 2013 to 2023.The database was established by Excel 2021,and the experimental animal strain,gender,modeling method,detection index,and application category were sorted out.The characteristics of the CNV animal model were analyzed.Result:After comparative analysis,it was found that the animal strains were Sprague-Dawley rats(87 times,29.49%)and New Zealand white rabbits(52 times,17.63%).Male animals were recommended.Most modeling methods for efficacy verification and mechanism studies were the alkali burn method.Index detection methods included apparent index observation,histopathological detection,immunohistochemistry(IHC),Western blot,and various polymerase chain reaction(PCR)tests.Detection indexes included apparent indication,corneal histopathology,CNV regulation,etc.Conclusion:The CNV model of SD rats induced by the alkali burn method is recommended for model replication,and the indexes are mainly selected from the growth of CNV,corneal histopathological test,and vascular endothelial growth factor(VEGF)-related test.In addition,according to the demand,the corneal apparent indication and the basic indexes related to the regulation of CNV,such as vascular endothelial growth factor receptor 2(VEGFR2),basic fibroblast growth factor(bFGF),and secretogranin III(Scg3)are also selected.Clinical treatment of CNV relies on anti-inflammatory drugs and anti-VEGF drugs,and there is a lack of application of traditional Chinese medicine(TCM),so the model needs to be improved by adding elements of TCM syndromes.