岩藻多糖对大鼠氧化应激的缓解作用研究

Alleviating effect of fucoidan on oxidative stress in rats

为了探讨岩藻多糖对大鼠氧化应激的缓解作用,选择28只8周龄SD雄性大鼠,随机分为3个处理组,分别为对照组(n=10)注射生理盐水和饲喂维持日粮;氧化应激组(n=9)腹腔注射10 mg/kg BW的敌草快和饲喂维持日粮;氧化应激与岩藻多糖联合处理组(n=9)注射10 mg/kg BW的敌草快(diquat)并饲喂添加250 mg/kg岩藻多糖的试验日粮。试验第12 d,氧化应激组和联合处理组大鼠腹腔注射敌草快,建立氧化应激模型。试验结束后,采集血液、肝脏和脾脏组织样品。结果显示,饲喂岩藻多糖缓解氧化应激诱导的大鼠血清中天门冬氨酸氨基转移酶(aspartate aminotransferase, AST)活性的升高,降低肝脏中丙二醛(malondialdehyde, MDA)含量,并逆转由氧化应激诱导的肝脏组织中核转录因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)、超氧化物歧化酶2(superoxide dismutase 2,SOD2)和谷胱甘肽过氧化物酶(glutathione peroxidase, GPx)基因表达的下调,从而提高总抗氧化能力(total antioxidant capacity, T-AOC)和GPx活性。此外,饲喂岩藻多糖能够逆转氧化应激诱导的大鼠脾脏组织中Nrf2和GPx基因表达的下调,从而提高大鼠脾脏组织中T-AOC及SOD和GPx的活性。因此,岩藻多糖通过Nrf2途径调控大鼠肝脏和脾脏的抗氧化酶活性来缓解大鼠氧化应激。

The present study was conducted to investigate the relieving effect of fucoidan on oxidative stress in rats.A total of 28 eight-week-old male SD rats were divided into three groups:control group(n=10)injected intraperitoneally with saline and feeding basal diet,oxidative stress group(n=9)injected intraperitoneally with 10 mg/kg BW diquat and feeding basal diet,fucoidan combined with oxidative stress treatment group(n=9)injected intraperitoneally with 10 mg/kg BW diquat and feeding diet supplemented with 250 mg/kg fucoidan.On the twelfth day of the trial,rats in oxidative stress and fucoidan combined with oxidative stress treatment groups were intraperitoneally injected diquat to establish oxidative stress model.At the end of the trial,the blood,liver and spleen tissue samples were collected.The results showed that dietary fucoidan relieved the increase of serum aspartate aminotransferase(AST)activity and decreased hepatic malondialdehyde(MDA)content of rats,and reversed the down-regulation of hepatic nuclear factor erythroid 2-related factor 2(Nrf2),superoxide dismutase 2(SOD2)and glutathione peroxidase(GPx)gene expression induced by oxidative stress,and improved the total antioxidant capacity(T-AOC)and GPx activity.In addition,dietary fucoidan reversed the down-regulation of splenic Nrf2 and GPx gene expression of rats induced by oxidative stress,and increased the T-AOC and activities of SOD and GPx.In conclusion,dietary fucoidan can alleviates oxidative stress in rats through its antioxidant activity via Nrf2 pathway.