MTA2、SET8蛋白在喉癌中的表达及与临床病理特征的关系

The Expression of MTA2 and SET8 Proteins in Laryngeal Carcinoma and Their Relationship with Clinicopathologic Features

目的探讨MTA2、SET8蛋白在喉癌中的表达及与临床病理特征的关系。方法收集喉癌患者205例作为研究对象,采用免疫组织化学染色法检测MTA2、SET8阳性蛋白,采用Real-time PCR检测MTA2、SET8 mRNA表达水平。分析MTA2、SET8蛋白表达与喉癌患者临床病理特征的关系。Spearson相关性分析MTA2蛋白与SET8蛋白表达之间的关系。结果喉癌组MTA2、SET8蛋白阳性表达率(56.10%、54.15%)显著高于癌旁组,差异均有统计学意义(χ^(2)=27.926,P<0.001;χ^(2)=28.277,P<0.001);喉癌组MTA2、SET8 mRNA表达水平高于癌旁组,差异均具有统计学意义(t=25.005,P<0.001;t=20.390,P<0.001)。喉癌组织中MTA2、SET8蛋白阳性表达与性别、年龄、是否吸烟、临床分型均无关(P>0.05),与淋巴结是否转移、病理分级、临床分期有关(P<0.05)。Spearson相关性分析显示,MTA2蛋白与SET8蛋白表达呈现正相关(γ=0.477,P<0.05)。结论MTA2、SET8蛋白在喉癌中表达明显上升,且与喉癌的发展密切相关,预示其可能成为诊断分子的潜能。

Objective To investigate the expression of MTA2 and SET8 proteins in laryngeal carcinoma and their relationship with clinicopathological features.Methods 205 patients with laryngeal cancer were collected as research objects,and MTA2 and SET8 positive proteins were detected by immunohistochemical staining,and the mRNA expression levels of MTA2 and SET8 were detected by Real-time PCR.To analyze the relationship between the expression of MTA2 and SET8 proteins and clinicopathological features of laryngeal carcinoma patients.Spearson correlation analysis of MTA2 protein and SET8 protein expression.Results The positive expressions of MTA2 and SET8 proteins in the laryngeal cancer group were significantly higher than those in the paracancer group by 56.10%and 54.15%,with statistical significance(χ^(2)=27.926,P<0.001;χ^(2)=28.277,P<0.001).The mRNA expression levels of MTA2 and SET8 in laryngeal cancer group were higher than those in adjacent cancer group,and the differences were statistically significant(t=25.005,P<0.001;t=20.390,P<0.001).The positive expression of MTA2 and SET8 proteins in laryngeal cancer tissues was not related to gender,age,smoking or not,and clinical type(P>0.05),but were related to lymph node metastasis,pathological grade and clinical stage(P<0.05).Spearson correlation analysis showed that the expression of MTA2 protein was positively correlated with that of SET8 protein(γ=0.477,P<0.05).Conclusion The expression of MTA2 and SET8 proteins increased significantly in laryngeal cancer,and they are closely related to the development of laryngeal cancer,indicating that they may be potential diagnostic molecules.