miR-191在宫颈癌组织中的表达及其临床意义

Expression of miR-191 in Cervical Carcinoma and Its Clinical Significance

目的探讨miR-191在宫颈癌组织内的表达及临床意义。方法选取75例宫颈癌患者作为研究对象,均采用以手术为主的治疗方案。术中采集肿瘤组织标本,并采集癌旁正常组织标本。采用实时荧光定量PCR法检测miR-191在肿瘤组织及正常组织中的表达水平,并分析miR-191表达与临床病理特征及预后间的关系。结果肿瘤组织内miR-191表达为(1.98±0.15),高于正常组织的(0.89±0.12),差异有统计学意义(P<0.05)。肿瘤大小≥4 cm、高危HPV感染、病理分级G3、肿瘤分期Ⅲ期、淋巴结转移及肌层浸润≥1/2的宫颈癌患者miR-191表达水平高于肿瘤大小<4 cm、无高危HPV感染、病理分级G1~G2、肿瘤分期Ⅰ~Ⅱ期、无淋巴结转移、肌层浸润<1/2患者,差异有统计学意义(P<0.05)。随访3年,75例宫颈癌患者,存活61例,存活率为81.33%(61/75);存活患者miR-191表达为(1.82±0.14),低于死亡患者的(2.49±0.22),差异有统计学意义(P<0.05)。结论miR-191在宫颈癌组织内呈较高表达,且表达与肿瘤大小、病理分级、肿瘤分期及淋巴结转移等关系密切,能够作为早期辅助治疗及预后评估的重要指标。

Objective To investigate the expression and clinical significance of miR-191 in cervical cancer.Methods 75 patients with cervical cancer were selected as the study subjects,and all of them were treated with surgery.The expression of miR-191 in tumor tissues and normal tissues was detected by real-time fluorescence quantitative PCR.The relationship between miR-191 expression and clinicopathological characteristics and prognosis was analyzed.Results The expression of miR-191 in tumor tissue was(1.98±0.15),which was significantly higher than that in normal tissue(0.89±0.12)(P<0.05);The expression level of miR-191[(2.14±0.18),(2.09±0.17),(2.23±0.21),(2.18±0.19),(2.12±0.49),(2.25±0.22)]in cervical cancer patients with tumor size≥4 cm,high-risk HPV infection,pathological grade G3,tumor stage Ⅲ,lymph node metastasis and myometrial invasion≥1/2 was higher than that of tumor size<4 cm,no high-risk HPV infection,pathological grade G1~G2,tumor stage Ⅰ~Ⅱ,no lymph node metastasis The difference was statistically significant(P<0.05);Following up for 3 years,61 of 75 patients with cervical cancer survived,with a survival rate of 81.33%(61/75);The expression of miR-191 in the living patients was(1.82±0.14),lower than that in the dead patients(2.49±0.22),and the difference was statistically significant(P<0.05).Conclusion miR-191 is highly expressed in cervical cancer tissue,and its expression is closely related to tumor size,pathological grade,tumor stage and lymph node metastasis.It can be used as an important indicator for early adjuvant treatment and prognosis evaluation.