补肾活血方对复发性流产患者血清VEGF、HIF-1α、TGF-β_(1)、sflt-1影响研究

Study pf Bushen Huoxue recipe on serum of VEGF/HIF-1α/TGF-β_(1)/sfit-1 of posi⁃tive antiphospholipid antibodies Patients

目的 研究补肾活血方对APL阳性RSA患者外周血VEGF、HIF-1α、TGF-β_(1)、sflt-1、miR-423-5p蛋白表达量影响。方法 选取APL阳性RSA患者60例,分别于孕前给予药物调理。其中观察组服用补肾活血方,对照组服用肠溶阿司匹林,均治疗3个月。两组治疗前后分别检测血清VEGF、HIF-1α、TGF-β_(1)、sflt-1、miR-423-5p蛋白表达及中医证候积分。结果 两组治疗后VEGF蛋白表达均显著升高;与对照组比较,观察组VEGF蛋白表达量显著高于对照组;两组治疗前后,HIF-1α蛋白表达量均无显著性差异;治疗后两组TGF-β1蛋白表达显著升高,与对照组比较,观察组TGF-β1蛋白表达显著升高;治疗后两组sflt-1蛋白表达显著降低;与对照组比较,观察组sflt-1蛋白表达显著降低。两组治疗后miR-423-5p相对表达量均降低,观察组优于对照组(P<0.05)。观察组中医证候疗效比较,治疗组优于对照组(P<0.05)。结论 补肾活血方应用于RSA患者孕前调理,可以通过上调VEGF、TGF-β_(1)蛋白表达,下调sflt-1、miR-423-5p蛋白表达等途径,改善APL对子宫内膜的不良影响;同时可有效降低中医证候积分,从而降低再次妊娠后流产的发生率。

Objective To study the effect of Bushen Huoxue Formula on peripheral blood VEGF,HIF-1α,TGF in APL positive RSA patients-β_(1).The expression levels of sflt-1 and miR-423-5p proteins are affected.Methods Sixty APL positive RSA patients were selected and administered medication before pregnancy.The observation group received the tonifying kidney and ac⁃tivating blood formula,while the control group received enteric coated aspirin,both receiving treatment for 3 months.Serum VEGF,HIF-1α,and TGF were measured before and after treatment in both groups-β_(1).Expression of sflt-1 and miR-423-5p proteins and integration of traditional Chinese medicine syndromes.Results The expression of VEGF protein was significantly increased in both groups after treatment;Compared with the control group,the expression of VEGF protein in the observation group was significantly higher than that in the control group;HIF-1 before and after treatment in both groupsαThere was no sig⁃nificant difference in protein expression levels;Two groups of TGF after treatment-β_(1).The protein expression was significantly increased,and compared with the control group,the observation group had TGF-β_(1).The protein expression significantly in⁃creased;After treatment,the expression of sflt-1 protein significantly decreased in both groups;Compared with the control group,the expression of sflt-1 protein in the observation group was significantly reduced.After treatment,the relative expression of miR-423-5p decreased in both groups,and the observation group was better than the control group(P<0.05).The obser⁃vation group compared the efficacy of traditional Chinese medicine syndromes,and the treatment group was better than the control group(P<0.05).Conclusion Bushen Huoxue Recipe can be used to regulate RSA patients before pregnancy by up regulating VEGF and TGF-β_(1) protein expression,downregulation of sflt-1,miR-423-5p protein expression,and other pathways to im⁃prove the adverse effects of APL on the endometrium;At the same time,it can effectively reduce the traditional Chinese medicine syndrome score and reduce the incidence of miscarriage after another pregnancy.