血清miRNA-199a-5p、α-1-B-糖蛋白反义RNA1水平与脑胶质瘤患者术后复发的关系

Levels of serum miRNA 199a-5p and alpha-1-B-glycoprotein antisense RNA-1 in patients with brain glioma and their relationship with postoperative recurrence

目的检测脑胶质瘤患者的血清微小RNA(miRNA)-199a-5p、α-1-B-糖蛋白反义RNA1(A1BG-AS1)水平,并分析其与患者术后复发的关系。方法选取94例接受手术治疗的脑胶质瘤患者和84例健康体检者,分别作为研究组和对照组。术后所有脑胶质瘤患者均随访2年,依据是否复发分为复发组(n=71)和非复发组(n=23)。采用聚合酶链反应(PCR)检测血清miRNA-199a-5p、A1BG-AS1水平,比较研究组和对照组、复发组和非复发组的血清miRNA-199a-5p、A1BG-AS1水平。采用Spearman相关分析法分析血清miRNA-199a-5p、A1BG-AS1水平与脑胶质瘤患者术后复发的相关性。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),分析血清miRNA-199a-5p、A1BG-AS1单独及联合检测对脑胶质瘤患者术后复发的评估价值。结果研究组患者血清miRNA-199a-5p水平明显低于对照组,血清A1BG-AS1水平明显高于对照组,差异均有统计学意义(P﹤0.01)。复发组患者血清miRNA-199a-5p水平低于非复发组,血清A1BG-AS1水平高于非复发组,差异均有统计学意义(P﹤0.05)。血清miRNA-199a-5p水平与脑胶质瘤患者术后复发呈负相关(r=-0.382,P﹤0.05),血清A1BG-AS1水平与脑胶质瘤患者术后复发呈正相关(r=0.423,P﹤0.05)。ROC曲线显示,血清miRNA-199a-5p、A1BG-AS1联合检测评估脑胶质瘤患者术后复发的AUC和灵敏度均高于二者单独检测。结论脑胶质瘤患者血清miRNA-199a-5p水平降低,血清A1BG-AS1水平升高,且其表达水平与脑胶质瘤患者术后复发有关,联合检测对术后复发具有较好的评估价值。

Objective To detect the serum microRNA(miRNA)-199a-5p and alpha-1-B-glycoprotein antisense RNA 1(A1BG-AS1)levels in patients with brain glioma,and their relationship with postoperative recurrence of patients.MethMeth-od A total of 94 patients with brain glioma who received surgerical treatment and 84 healthy subjects were selected as study group and control group,respectively.All patients with brain glioma were followed up for 2 years after surgery,and were divided into recurrence group(n=71)and non-recurrence group(n=23)according to whether recurrence or not.The serum miRNA-199a-5p and A1BG-AS1 levels were detected by polymerase chain reaction(PCR),and serum miRNA-199a-5p and A1BG-AS1 levels were compared between the study group and the control group,and between the recur-rence group and non-recurrence group.Spearman correlation analysis was used to analyze the correlation between serum miRNA-199a-5p,A1BG-AS1 levels and postoperative recurrence of brain glioma patients.The receiver operating charac-teristic(ROC)curve was plotted and the area under the curve(AUC)was calculated to analyze the value of serum miR-NA-199a-5p and A1BG-AS1 single and combined detection in evaluating postoperative recurrence of patients with brain glioma.Result The serum miRNA-199a-5p level in the study group was significantly lower than that in the control group,and the serum A1BG-AS1 level was significantly higher than that in the control group,and the differences were statistically significant(P<0.01).The serum miRNA-199a-5p level in the recurrence group was lower than that in the non-recurrence group,and the serum A1BG-AS1 level was higher than that in the non-recurrence group,and the differences were statistically significant(P<0.05).Serum miRNA-199a-5p level was negatively correlated with postoperative recur-rence of brain glioma patients(r=-0.382,P<0.05),and serum A1BG-AS1 level was positively correlated with postopera-tive recurrence of glioma patients(r=0.423,P<0.05).ROC curve showed that the AUC and sensitivity of serum miRNA-199a-5p and A1BG-AS1 combined detection in evaluating postoperative recurrence of brain glioma patients were higher than those of single detection.Conclusion The level of serum miRNA-199a-5p is decreased and the level of serum A1BG-AS1 is increased in patients with brain glioma,and there expression levels are associated with postoperative recur-rence of brain glioma patients,combined detection has high value in evaluating postoperative recurrence.