烟草废弃物提取物的双水相萃取及其化妆品功效研究

Two-phase extraction of tobacco waste extracts and their cosmetic efficacy

采用双水相萃取法制备烟草废弃物提取物,探究不同萃取条件对其中上相多酚得率的影响,并探讨其抗氧化活性、紫外吸收特性和细胞毒性。结果表明:当料液质量比为1∶60,搅拌时间为30 min,(NH4)2SO4质量分数为23.3%,乙醇质量分数为23.5%时,上相多酚得率最高,为48.59 mg/g;该萃取条件下,烟草废弃物提取物具有较好的抗氧化活性,当质量浓度为2 mg/mL时,对DPPH自由基清除率达98%,对ABTS自由基清除率达78%;当提取物质量浓度为0.5 mg/mL时,具有较好的紫外吸收特性,有一定的光防护功效;当提取物质量浓度在0~1.2 mg/mL范围内时,对Hacat细胞无细胞毒性,有望成为天然无刺激的功效型化妆品原料。

A two-phase aqueous extraction method was established to prepare tobacco waste extracts.The effects of different extraction conditions on the yield of upper phase polyphe nols were studied.The antioxidant activity and UV absorption chaoracteristic were also discussed.The results indicated when the solid-liquid ratio of tobacco waste was 1∶60,the stirring time was 30 min,the mass fraction of(NH4)2SO4 was 23.3%and the ethanol was 23.5%,the extraction rate of upper phase polyphenols was the highest,and the content of polyphenol was 48.59 mg/g.The tobacco waste extracts showed good antioxidant activity,and the scavenging rate of DPPH free radical was 98%and ABTS free radical was 78%at the mass concentration of 2 mg/mL.When the mass concentration of the extracts was 0.5 mg/mL,the extracts had good UV absorption and a certain effect of light protection.When the mass concentration of the extracts was within the range of 0~1.2 mg/mL,the extracts had no cytotoxicity to HaCat cells and were expected to become a natural and non-irritating functional cosmetic raw material.