UBE2L6在卵巢癌中的表达及其预后价值

Expression and prognostic value of UBE2L6 in ovarian cancer

目的探讨UBE2L6基因在卵巢癌组织中的表达,并分析其与患者预后的关系。方法收集2014年7月至2022年2月在山西白求恩医院住院治疗的150例卵巢癌患者及因子宫腺肌症进行全子宫+双侧附件切除的患者25例,采用免疫组化SP法检测卵巢组织中UBE2L6蛋白的表达情况,比较UBE2L6蛋白在卵巢癌组织与正常卵巢组织中的表达差异;并分析UBE2L6蛋白表达与卵巢癌患者临床病理特征之间的关系;通过χ2检验、COX风险回归分析筛选影响卵巢癌患者无进展生存期的独立危险因素,并采用Kaplan-Meier法绘制生存曲线。基于基因表达在线数据库分析UBE2L6表达与卵巢癌临床病理指标相关性,通过Kaplan-Meier数据库检索UBE2L6表达与卵巢癌预后的相关性;利用京都基因与基因组百科全书富集分析预测基因功能;通过肿瘤免疫估计资源(TIMER)数据库分析UBE2L6在卵巢癌高表达与局部免疫浸润的相关性。结果与正常卵巢组织相比,UBE2L6在卵巢癌中高表达(P<0.05),与患者不良预后相关;COX风险回归模型分析显示,UBE2L6高表达是卵巢癌无进展生存期的独立危险因素(P<0.05)。基因富集分析显示UBE2L6可能通过炎症通路影响卵巢癌的发生发展及预后;TIMER数据库表明UBE2L6表达水平与B细胞(P=9.87×10^(-11))、CD8+T细胞(P=9.64×10^(-13))、中性粒细胞(P=2.13×10-22)、树突状细胞(P=6.50×10-15)及CD4^(+)T细胞(P=3.51×10^(-4))呈正相关,与肿瘤纯度呈负相关(P=7×10-8)。结论UBE2L6可能通过影响免疫细胞进而导致不良预后,其可能是卵巢癌治疗的潜在靶点。

Objective To explore the expression of UBE2L6 gene in ovarian cancer and its relationship with prognosis by bioinformatics method.Methods A total of 150 patients with ovarian cancer hospitalized in Shanxi Bethune Hospital from July 2014 to February 2022 and 25 patients undergoing total hysterectomy plus bilateral adenectomy due to adenomyosis were collected.Immunohistochemical SP method was used to detect the expression of UBE2L6 protein.The expression of UBE2L6 protein was compared between ovarian cancer and normal ovarian tissue.The relationships between UBE2L6 protein expression and clinicopathological features of ovarian cancer patients were analyzed.Independent risk factors affecting progression-free survival of ovarian cancer patients were screened byχ2 test and COX risk regression analysis,and survival curves were plotted by Kaplan-Meier method.The correlation between UBE2L6 expression and clinicopathological indicators of ovarian cancer was analyzed based on online gene expression database,and the correlation between UBE2L6 expression and prognosis of ovarian cancer was retrieved through Kaplan-Meier database.Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed to predict gene function.The correlation between high expression of UBE2L6 in ovarian cancer and local immune invasion was analyzed through the Tumor Immune Estimation Resource(TIMER)database.Results Compared with normal ovarian tissues,UBE2L6 expression was increased in ovarian cancer tissues(P<0.05),and it was associated with the poor prognosis.COX regression analysis showed that UBE2L6 high expression was an independent risk factor for progression-free survival of ovarian cancer patients(P<0.05).Gene enrichment analysis showed that UBE2L6 may affect the development and prognosis of ovarian cancer through inflammatory pathways.The TIMER database showed that the expression level of UBE2L6 was positively correlated with B cells(P=9.87×10^(-11)),CD8+T cells(P=9.64×10^(-13)),neutrophils(P=2.13×10-22),dendritic cells(P=6.50×10-15),CD4^(+)T cells(P=3.51×10^(-4)),and negatively correlated with tumor purity(P=7×10-8).Conclusion UBE2L6 may lead to poor prognosis by affecting immune cells,which may be a potential target for ovarian cancer treatment.