芹菜不同品种生长时期挥发性有机物质成分分析

Analysis of Volatile Organic Compounds in Different Varieties of Celery at Different Growth Stages

【目的】探究芹菜挥发性有机物质在不同品种及不同生长时期的变化规律和分布特征。【方法】选取了不同叶柄颜色的芹菜品种‘特级白芹王’‘津南实芹2号’和‘黄嫩西芹’为试验材料,利用顶空固相微萃取(HS-SPME)技术对芹菜挥发物进行提取,结合气相色谱-质谱仪(GC-MS)对挥发物质进行测定与比较分析。【结果】3个芹菜品种共鉴定出161种化合物,其中‘津南实芹2号’品种整体挥发物含量最高;‘特级白芹王’和‘津南实芹2号’品种在成熟期挥发性有机物含量最高,而‘黄嫩西芹’挥发性有机物含量最高的时期则为快速生长期,表明3个品种在不同时期间挥发物质种类和含量上存在差异。基于OPLS-DA模型发现,(-)-camphene、2,4-dimethoxyamphetamine、3-methylheptyl acetate、(+)-camphene分别在‘特级白芹王’成熟期、‘津南实芹2号’幼苗期、‘津南实芹2号’成熟期、‘黄嫩西芹’快速生长期含量较高,这些化合物可作为区分3个品种和发育时期的重要标志性化合物。【结论】利用HSSPME-GC-MS技术明确了不同时期芹菜品种间挥发性有机物的分布和变化规律,为后续利用不同种质资源的芹菜及开展香气品质的遗传改良提供参考。

【Objective】This study aims to investigate the patterns of variation and distribution characteristics of volatile organic compounds(VOCs)in celery across different varieties and growth stages.【Method】For this study,three celery varieties with different petiole colors—'Teji Baiqinwang''Jinnan Shiqin No.2'and'Huangnen Xiqin'—were selected as the experimental materials.The volatile organic compounds in celery were extracted using the headspace solid-phase microextraction(HS-SPME)technique,and gas chromatography-mass spectrometry(GC-MS)was utilized for the determination and comparative analysis of these VOCs.【Result】The three celery varieties were found to contain a combined total of 161 compounds.Among them,the variety'Jinnan Shiqin No.2'exhibited the highest overall content of VOCs.Both the varieties'Teji Baiqinwang'and'Jinnan Shiqin No.2'displayed the highest levels of VOCs at the mature stage,whereas the variety'Huangnen Xiqin'exhibited higher content at the rapid growth stage.These findings indicate variations in the quantities and patterns of VOCs among the three celery varieties at different growth stages.According to the OPLS-DA model,it was determined that(-)-camphene,2,4-dimethoxyamphetamine,3-methylheptyl acetate,and(+)-camphene exhibit higher concentrations in the mature stage of'Teji Baiqinwang',the seeding stage and mature stage of'Jinnan Shiqin No.2',and the rapid growth stage of'Huangnen Xiqin',respectively.These compounds can serve as important markers for distinguishing between different varieties and growth stages.【Conclusion】In this study,HS-SPME-GC-MS technology was used to clarify the distribution and change rules of VOCs among celery varieties in different growth periods,which will provide a reference for the future utilization of celery with different germplasm resources and for the genetic improvement of aroma quality.